FAM134A Polyklonaler Antikörper

FAM134A Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 24650-1-PBS

Synonyme

C2orf17, MAG2, Protein FAM134A, Reticulophagy regulator 2, RETREG2

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  WB analysis using 24650-1-AP (same clone as 24650-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 24650-1-AP (FAM134A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24650-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 24650-1-AP (same clone as 24650-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24650-1-AP (FAM134A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24650-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 24650-1-AP (same clone as 24650-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24650-1-AP (FAM134A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24650-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 24650-1-AP (same clone as 24650-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using FAM134A antibody (24650-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 24650-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

24650-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA FAM134A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FAM134A fusion protein Ag20025
• Vollständiger Name: family with sequence similarity 134, member A
• Berechnetes Molekulargewicht: 543 aa, 58 kDa
• Beobachtetes Molekulargewicht: 70-80 kDa
• GenBank-Zugangsnummer: BC002420
• Gene symbol: FAM134A
• Gene ID (NCBI): 79137
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The protein family with sequence similarity 134 (FAM134) consists of three proteins, FAM134B, FAM134A, and FAM134C, which share the same LIR amino acid sequence. FAM134 reticulon family proteins (FAM134A, FAM134B, and FAM134C) are ER-phagy receptors with structural similarities and nonredundant functions. The protein family with sequence similarity 134 (FAM134). Fam134A (also known as metastasis-associated genes 2, MAG-2) is widely expressed in various types of tissues including tumors, and is considered to be closely correlated with tumor proliferation, invasion, and metastasis.