FAM134A Rekombinanter Antikörper

FAM134A Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

250097C7

Kat-Nr. : 85791-4-RR

Synonyme

C2orf17, MAG2, Protein FAM134A, Reticulophagy regulator 2, RETREG2

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  WB analysis using 85791-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85791-4-RR (FAM134A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 85791-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 85791-4-RR (FAM134A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 85791-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 85791-4-RR (FAM134A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 85791-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85791-4-RR against Human FAM134A were performed. The affinity constant is 3.17 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, HEK-293-Zellen, U2OS-Zellen
• Erfolgreiche Detektion in IHC: Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85791-4-RR bindet in WB, IHC, ELISA FAM134A und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: FAM134A fusion protein Ag20025
• Vollständiger Name: family with sequence similarity 134, member A
• Berechnetes Molekulargewicht: 543 aa, 58 kDa
• Beobachtetes Molekulargewicht: 70-80 kDa
• GenBank-Zugangsnummer: BC002420
• Gene symbol: FAM134A
• Gene ID (NCBI): 79137
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The protein family with sequence similarity 134 (FAM134) consists of three proteins, FAM134B, FAM134A, and FAM134C, which share the same LIR amino acid sequence. FAM134 reticulon family proteins (FAM134A, FAM134B, and FAM134C) are ER-phagy receptors with structural similarities and nonredundant functions. The protein family with sequence similarity 134 (FAM134). Fam134A (also known as metastasis-associated genes 2, MAG-2) is widely expressed in various types of tissues including tumors, and is considered to be closely correlated with tumor proliferation, invasion, and metastasis.