FAM136A Polyklonaler Antikörper

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• KD/KO Validated

FAM136A Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 30845-1-AP

Synonyme

Protein FAM136A

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  WB analysis using 30845-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30845-1-AP (FAM136A antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 30845-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30845-1-AP (FAM136A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 30845-1-AP

  WB result of FAM136A antibody (30845-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FAM136A transfected MCF-7 cells.

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  IHC staining of mouse testis using 30845-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30845-1-AP (FAM136A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat testis using 30845-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 30845-1-AP (FAM136A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 30845-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 30845-1-AP (FAM136A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 30845-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using FAM136A antibody (30845-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, Jurkat-Zellen, MCF-7-Zellen, Maushodengewebe, Rattenhodengewebe, U2OS-Zellen
• Erfolgreiche Detektion in IHC: human ovary cancer tissue, Maushodengewebe, Rattenhodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

30845-1-AP bindet in WB, IHC, IF/ICC, ELISA FAM136A und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FAM136A fusion protein Ag34058
• Vollständiger Name: family with sequence similarity 136, member A
• Beobachtetes Molekulargewicht: 15 kDa
• GenBank-Zugangsnummer: BC014975
• Gene symbol: FAM136A
• Gene ID (NCBI): 84908
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FAM136A is an evolutively conserved nuclear gene encoding a mitochondrial protein whose specific function is unknown, but which is commonly found in neurosensory epithelial cells, where it plays a role in the electron transport chain of respiration (PMID: 37461313). FAM136A, with a molecular mass of 16-kDa, is normally expressed in the cytoplasm and has been linked to familial Meniere's disease.

Publikationen

Species	Application	Title
human	WB	FEBS Open Bio; FAM136A depletion induces mitochondrial stress and reduces mitochondrial membrane potential and ATP production; Authors - Yushi Otsuka