FAM136A Rekombinanter Antikörper

FAM136A Rekombinant Antikörper für WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241433D12

Kat-Nr. : 84150-2-PBS

Synonyme

241433D12, Protein FAM136A

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  WB analysis using 84150-2-RR (same clone as 84150-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84150-2-RR (FAM136A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84150-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 84150-2-RR (same clone as 84150-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84150-2-RR (FAM136A antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84150-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 84150-2-RR (same clone as 84150-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84150-2-RR (FAM136A antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84150-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84150-2-RR (same clone as 84150-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FAM136A antibody (84150-2-RR, Clone: 241433D12 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84150-2-PBS in a different storage buffer formulation.

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  FC experiment of U2OS using 84150-2-RR (same clone as 84150-2-PBS)

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug FAM136A Recombinant antibody (84150-2-RR, Clone:241433D12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84150-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01066-3

  Cytometric bead array standard curve of MP01066-3, FAM136A Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84150-2-PBS. Detection antibody: 84150-5-PBS. Standard: Ag34058. Range: 0.313-40 ng/mL.

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  Affinity and Kinetic Characterization of 84150-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84150-2-RR against Human FAM136A were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

84150-2-PBS bindet in WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA FAM136A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: FAM136A fusion protein Ag34058
• Vollständiger Name: family with sequence similarity 136, member A
• Beobachtetes Molekulargewicht: 12 kDa
• GenBank-Zugangsnummer: BC014975
• Gene symbol: FAM136A
• Gene ID (NCBI): 84908
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FAM136A is an evolutively conserved nuclear gene encoding a mitochondrial protein whose specific function is unknown, but which is commonly found in neurosensory epithelial cells, where it plays a role in the electron transport chain of respiration (PMID: 37461313). FAM136A, with a molecular mass of 16-kDa, is normally expressed in the cytoplasm and has been linked to familial Meniere's disease.