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FAM162A Polyklonaler Antikörper

FAM162A Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 32034-1-PBS

Synonyme

C3orf28, DC16, E2IG5, E2-induced gene 5 protein, FWP001

Filter:
  • Western Blot (WB) analysis of various lysates using FAM162A Polyclonal antibody (32034-1-AP)

    WB analysis using 32034-1-AP (same clone as 32034-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 32034-1-AP (FAM162A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 32034-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using FAM162A Polyclonal antibody (32034-1-AP)

    IHC staining of mouse colon using 32034-1-AP (same clone as 32034-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 32034-1-AP (FAM162A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32034-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using FAM162A Polyclonal antibody (32034-1-AP)

    IHC staining of mouse colon using 32034-1-AP (same clone as 32034-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 32034-1-AP (FAM162A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32034-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using FAM162A Polyclonal antibody (32034-1-AP)

    IF Staining of A431 using 32034-1-AP (same clone as 32034-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using FAM162A antibody (32034-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 32034-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

32034-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA FAM162A und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen FAM162A fusion protein Ag36569
Vollständiger Name family with sequence similarity 162, member A
Berechnetes Molekulargewicht 154 aa, 17 kDa
Beobachtetes Molekulargewicht17 kDa
GenBank-ZugangsnummerBC015060
Gene symbol FAM162A
Gene ID (NCBI) 26355
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Family with sequence similarity 162 member A (FAM162A, also known as E2IG5, HGTD-P, and C3orf28) is a mitochondrial apoptotic protein, which is known as an HIF-1 alpha-responsive pro-apoptotic molecule (PMID: 15082785; 16698020; 22776087). When FAM162A was overexpressed, the hypoxia signal was sent directly to the mitochondria and caused cell death (PMID: 16698020).