FAM171A2 Polyklonaler Antikörper

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• KD/KO Validated

FAM171A2 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 20836-1-AP

Synonyme

Protein FAM171A2

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  WB analysis of mouse liver using 20836-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 20836-1-AP (FAM171A2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 20836-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 20836-1-AP (FAM171A2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 20836-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20836-1-AP (FAM171A2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 20836-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20836-1-AP (FAM171A2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 20836-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20836-1-AP (FAM171A2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 20836-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 20836-1-AP (FAM171A2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 20836-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 20836-1-AP (FAM171A2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 20836-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20836-1-AP (FAM171A2 antibody) at dilution of 1:200 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, HepG2-Zellen
• Erfolgreiche Detektion in IHC: Maus-Cerebellum-Gewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

20836-1-AP bindet in WB, IHC, IF, ELISA FAM171A2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FAM171A2 fusion protein Ag14909
• Vollständiger Name: family with sequence similarity 171, member A2
• Berechnetes Molekulargewicht: 826 aa, 87 kDa
• Beobachtetes Molekulargewicht: 65-75 kDa
• GenBank-Zugangsnummer: BC030200
• Gene symbol: FAM171A2
• Gene ID (NCBI): 284069
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FAM171A2 is a key regulator of progranulin expression and modifies the risk of multiple neurodegenerative diseases. In addition, FAM171A2 was considerably expressed in the vascular endothelium and microglia, which are rich in Progranulin (PGRN) (PMID: 33087363).

Publikationen

Species	Application	Title
mouse	WB,IHC,IF	Sci Adv; The FAM171A2 gene is a key regulator of progranulin expression and modifies the risk of multiple neurodegenerative diseases.; Authors - Wei Xu ; KD Validated