FAM175A Polyklonaler Antikörper

FAM175A Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 14366-1-AP

Synonyme

UNQ496/PRO1013, Coiled-coil domain-containing protein 98, CCDC98, BRCA1-A complex subunit Abraxas 1, ABRAXAS1

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  WB analysis using 14366-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 14366-1-AP (FAM175A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 14366-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 14366-1-AP (FAM175A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 14366-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 14366-1-AP (FAM175A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 14366-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 14366-1-AP (FAM175A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 14366-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using FAM175A antibody (14366-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, HeLa-Zellen, humanes Hodengewebe, Jurkat-Zellen, MCF-7-Zellen, Raji-Zellen
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen, MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

14366-1-AP bindet in WB, IF/ICC, ELISA FAM175A und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FAM175A fusion protein Ag5688
• Vollständiger Name: family with sequence similarity 175, member A
• Berechnetes Molekulargewicht: 47 kDa
• Beobachtetes Molekulargewicht: 46 kDa
• GenBank-Zugangsnummer: BC039573
• Gene symbol: FAM175A
• Gene ID (NCBI): 84142
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The family with sequence similarity 175 member A gene (FAM175A; also known as ABRAXAS1, CCDC98 and ABRA1), a member of the DNA repair family, contributes to the BRCA1 (BRCA1 DNA repair associated)-dependent DNA damage response and is associated with age at natural menopause.

Publikationen

Species	Application	Title
human	WB	Nucleic Acids Res; RAP80 phase separation at DNA double-strand break promotes BRCA1 recruitment; Authors - Caolitao Qin