COX20 Polyklonaler Antikörper

COX20 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 25752-1-AP

Synonyme

FAM36A, Cytochrome c oxidase assembly protein COX20, mitochondrial, FAM 36A

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  WB analysis using 25752-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 25752-1-AP (COX20 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 25752-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 25752-1-AP (COX20 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human prostate cancer using 25752-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 25752-1-AP (COX20 antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human endometrial cancer using 25752-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 25752-1-AP (COX20 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human endometrial cancer using 25752-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 25752-1-AP (COX20 antibody at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 25752-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using COX20 antibody (25752-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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  IF Staining of PC-3 using 25752-1-AP

  Immunofluorescent analysis of PC-3 cells using 25752-1-AP (COX20 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe, humanes Endometriumkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen, PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Produktinformation

25752-1-AP bindet in WB, IHC, IF/ICC, ELISA COX20 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: COX20 fusion protein Ag22520
• Vollständiger Name: family with sequence similarity 36, member A
• Berechnetes Molekulargewicht: 118 aa, 13 kDa
• Beobachtetes Molekulargewicht: ~16 kDa
• GenBank-Zugangsnummer: BC018519
• Gene symbol: COX20
• Gene ID (NCBI): 116228
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

COX20, also name as FAM36A, belongs to the COX20 family. Mutations in COX20 are a novel cause of recessively inherited.

Publikationen

Species	Application	Title
human	WB,IF	Cell; Ribosome ADP-ribosylation inhibits translation and maintains proteostasis in cancers.; Authors - Sridevi Challa
mouse	WB	Sci Transl Med; NIT2 dampens BRD1 phase separation and restrains oxidative phosphorylation to enhance chemosensitivity in gastric cancer; Authors - Ziyang Wang
human	WB	Sci Adv; A dual role for PSIP1/LEDGF in T cell acute lymphoblastic leukemia; Authors - Lisa Demoen
mouse	WB,IF	Sci Data; Bioinformatic analysis of membrane and associated proteins in murine cardiomyocytes and human myocardium.; Authors - Shin-Haw Lee
human	WB,IHC	Front Endocrinol (Lausanne); Novel prognostic features and personalized treatment strategies for mitochondria-related genes in glioma patients; Authors - Ji Wu