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  • KD/KO Validated

Piezo1 (extracellular domain) Polyklonaler Antikörper

Piezo1 (extracellular domain) Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (5)

Anwendung

WB, IHC, IF/ICC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(199)

Kat-Nr. : 15939-1-AP

Synonyme

FAM38A, PIEZO1, KIAA0233, Membrane protein induced by beta-amyloid treatment, Piezo-type mechanosensitive ion channel component 1



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHeLa-Zellen, hTERT-RPE1-Zellen, SGC-7901-Zellen
Erfolgreiche Detektion in IHChumanes Hautgewebe, humanes Hautkrebsgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCTHP-1-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:200-1:1000
Immunhistochemie (IHC)IHC : 1:50-1:500
Immunfluoreszenz (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

15939-1-AP bindet in WB, IHC, IF/ICC, CoIP, ELISA Piezo1 (extracellular domain) und zeigt Reaktivität mit human

Getestete Reaktivität human
In Publikationen genannte Reaktivitäthuman, Hausschwein, Hund, Maus, Ratte, Zebrafisch
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Piezo1 (extracellular domain) fusion protein Ag7791
Vollständiger Name family with sequence similarity 38, member A
Berechnetes Molekulargewicht 286 kDa
Beobachtetes Molekulargewicht 233-286 kDa
GenBank-ZugangsnummerBC008073
Gene symbol Piezo1/FAM38A
Gene ID (NCBI) 9780
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Mechanotransduction, the conversion of mechanical force into biological signals, is a fundamental physiologic process of mammalian cells that influences many critical processes including embryonic development, tactile, pain, and auditory sensation, regulation of vascular tone, flow sensing in the kidney, and muscle and tendon stretch. FAM38A, also known as Piezo1, has recently been identified as a mechanotransduction protein that gets involved in mechanosensation and stretch-activated cation channel activation. Piezo1 also plays a key role in epithelial cell adhesion by maintaining integrin activation through R-Ras recruitment to the ER. Mutations in the gene encoding Piezo1 are associated with hereditary xerocytosis. Piezo1 also regulates extrusion to maintain homeostatic epithelial cell numbers. This antibody was raised against the extracellular domain of human Piezo1. (PMID: 20016066, 28416610)

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for Piezo1 (extracellular domain) antibody 15939-1-APProtokoll herunterladen
IHC protocol for Piezo1 (extracellular domain) antibody 15939-1-APProtokoll herunterladenl
IF protocol for Piezo1 (extracellular domain) antibody 15939-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
zebrafishIF

Nature

Skin cells undergo asynthetic fission to expand body surfaces in zebrafish.

Authors - Keat Ying Chan
  • KO Validated
zebrafish,mouseWB

Nature

Crowding induces live cell extrusion to maintain homeostatic cell numbers in epithelia.

Authors - Eisenhoffer George T GT
  • KD Validated
humanWB

Nature

Piezo1 integration of vascular architecture with physiological force.

Authors - Jing Li
  • KD Validated
zebrafish,canineWB,IF

Nature

Mechanical stretch triggers rapid epithelial cell division through Piezo1.

Authors - S A Gudipaty
  • KO Validated
mouseIHC

Cell

RNA Sensing by Gut Piezo1 Is Essential for Systemic Serotonin Synthesis.

Authors - Erika Sugisawa
humanWB

Blood

Mechanosensation by endothelial PIEZO1 is required for leukocyte diapedesis.

Authors - ShengPeng Wang
  • KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Yao (Verified Customer) (09-24-2025)

I used this antibody for the kidney slides of wild-type mice, it showed positive staining that matches the expected pattern

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Kidney
Piezo1 (extracellular domain) Antibody Immunohistochemistry validation (1:100 dilution) in Kidney (Cat no:15939-1-AP)
FH

Hyun (Verified Customer) (07-23-2025)

This antibody works well for western blot.

  • Applications: Western Blot, Immunohistochemistry
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: rat brain tissue
FH

Jason (Verified Customer) (02-11-2025)

The antibody works well. However, due to the large protein size, obtaining sharp and clean Western blot bands is challenging for me. In fluorescence staining, the knockout cells were stained similarly to the control cells, suggesting non-specific staining in IF.

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1:500 for WB; 1:200 for IF
  • Cell Tissue Type: Human Dermal Microvascular Endothelial Cell
Piezo1 (extracellular domain) Antibody Western Blot,Immunofluorescence validation (1:500 for WB; 1:200 for IF dilution) in Human Dermal Microvascular Endothelial Cell (Cat no:15939-1-AP)
FH

Hyojin (Verified Customer) (01-19-2024)

I like the quality of antibody. Thank you for generating.

  • Applications: Western Blot, Immunohistochemistry
  • Primary Antibody Dilution: 1:200 for IF 1:1000 for WB
  • Cell Tissue Type: mouse/human
FH

Sammy (Verified Customer) (12-04-2023)

Ab detects a band at the correct size but the signal is weak. This works only when detected via HRP/Chemi. NIR-conjugated antibodies do not detect this band.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1 in 1000
  • Cell Tissue Type: HUVEC
Piezo1 (extracellular domain) Antibody Western Blot validation (1 in 1000 dilution) in HUVEC (Cat no:15939-1-AP)
FH

Xiaonan (Verified Customer) (02-01-2023)

We did not see the band for Piezol.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: mouse kidney
Piezo1 (extracellular domain) Antibody Western Blot validation (1:1000 dilution) in mouse kidney (Cat no:15939-1-AP)
FH

LUNFENG (Verified Customer) (01-27-2020)

GOOD

  • Applications: Immunofluorescence,
  • Cell Tissue Type: HEART
FH

Jie (Verified Customer) (01-23-2020)

Works with one band. Due to the large protein size, band does not always show sharp image.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Hela cells
FH

Canzhao (Verified Customer) (01-17-2020)

It worked in western blot and showed nice band.

  • Applications: Western Blot,
  • Cell Tissue Type: Mouse heart tissue
FH

Zeinab (Verified Customer) (09-27-2019)

Worked great, Clean and Specific

  • Applications: Western Blot, Immunofluorescence,
  • Cell Tissue Type: Human Cardiac Microvascular Endothelial cells