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Piezo1 (extracellular domain) Polyklonaler Antikörper
Piezo1 (extracellular domain) Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human und mehr (5)
Anwendung
WB, IHC, IF/ICC, CoIP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 15939-1-AP
Synonyme
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | HeLa-Zellen, hTERT-RPE1-Zellen, SGC-7901-Zellen |
Erfolgreiche Detektion in IHC | humanes Hautgewebe, humanes Hautkrebsgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Erfolgreiche Detektion in IF/ICC | THP-1-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
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Western Blot (WB) | WB : 1:200-1:1000 |
Immunhistochemie (IHC) | IHC : 1:50-1:500 |
Immunfluoreszenz (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
KD/KO | See 48 publications below |
WB | See 128 publications below |
IHC | See 48 publications below |
IF | See 100 publications below |
CoIP | See 6 publications below |
Produktinformation
15939-1-AP bindet in WB, IHC, IF/ICC, CoIP, ELISA Piezo1 (extracellular domain) und zeigt Reaktivität mit human
Getestete Reaktivität | human |
In Publikationen genannte Reaktivität | human, Hausschwein, Hund, Maus, Ratte, Zebrafisch |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Polyklonal |
Typ | Antikörper |
Immunogen | Piezo1 (extracellular domain) fusion protein Ag7791 |
Vollständiger Name | family with sequence similarity 38, member A |
Berechnetes Molekulargewicht | 286 kDa |
Beobachtetes Molekulargewicht | 233-286 kDa |
GenBank-Zugangsnummer | BC008073 |
Gene symbol | Piezo1/FAM38A |
Gene ID (NCBI) | 9780 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Antigen-Affinitätsreinigung |
Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Mechanotransduction, the conversion of mechanical force into biological signals, is a fundamental physiologic process of mammalian cells that influences many critical processes including embryonic development, tactile, pain, and auditory sensation, regulation of vascular tone, flow sensing in the kidney, and muscle and tendon stretch. FAM38A, also known as Piezo1, has recently been identified as a mechanotransduction protein that gets involved in mechanosensation and stretch-activated cation channel activation. Piezo1 also plays a key role in epithelial cell adhesion by maintaining integrin activation through R-Ras recruitment to the ER. Mutations in the gene encoding Piezo1 are associated with hereditary xerocytosis. Piezo1 also regulates extrusion to maintain homeostatic epithelial cell numbers. This antibody was raised against the extracellular domain of human Piezo1. (PMID: 20016066, 28416610)
Protokolle
PRODUKTSPEZIFISCHE PROTOKOLLE | |
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WB protocol for Piezo1 (extracellular domain) antibody 15939-1-AP | Protokoll herunterladen |
IHC protocol for Piezo1 (extracellular domain) antibody 15939-1-AP | Protokoll herunterladenl |
IF protocol for Piezo1 (extracellular domain) antibody 15939-1-AP | Protokoll herunterladen |
STANDARD-PROTOKOLLE | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
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Nature Skin cells undergo asynthetic fission to expand body surfaces in zebrafish.
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Nature Crowding induces live cell extrusion to maintain homeostatic cell numbers in epithelia.
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Nature Mechanical stretch triggers rapid epithelial cell division through Piezo1.
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Blood Mechanosensation by endothelial PIEZO1 is required for leukocyte diapedesis.
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Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Yao (Verified Customer) (09-24-2025) | I used this antibody for the kidney slides of wild-type mice, it showed positive staining that matches the expected pattern
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FH Hyun (Verified Customer) (07-23-2025) | This antibody works well for western blot.
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FH Jason (Verified Customer) (02-11-2025) | The antibody works well. However, due to the large protein size, obtaining sharp and clean Western blot bands is challenging for me. In fluorescence staining, the knockout cells were stained similarly to the control cells, suggesting non-specific staining in IF.
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FH Hyojin (Verified Customer) (01-19-2024) | I like the quality of antibody. Thank you for generating.
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FH Sammy (Verified Customer) (12-04-2023) | Ab detects a band at the correct size but the signal is weak. This works only when detected via HRP/Chemi. NIR-conjugated antibodies do not detect this band.
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FH Xiaonan (Verified Customer) (02-01-2023) | We did not see the band for Piezol.
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FH LUNFENG (Verified Customer) (01-27-2020) | GOOD
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FH Jie (Verified Customer) (01-23-2020) | Works with one band. Due to the large protein size, band does not always show sharp image.
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FH Canzhao (Verified Customer) (01-17-2020) | It worked in western blot and showed nice band.
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FH Zeinab (Verified Customer) (09-27-2019) | Worked great, Clean and Specific
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