FAM3C Monoklonaler Antikörper

FAM3C Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2E10D5

Kat-Nr. : 60282-1-PBS

Synonyme

2E10D5, GS3786, ILEI, Interleukin like EMT inducer, Interleukin-like EMT inducer

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  WB analysis of fetal human brain using 60282-1-Ig (same clone as 60282-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60282-1-Ig (FAM3C Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60282-1-PBS in a different storage buffer formulation.

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  IHC staining of human oesophagus cancer using 60282-1-Ig (same clone as 60282-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60282-1-Ig (FAM3C Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60282-1-PBS in a different storage buffer formulation.

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  IHC staining of human oesophagus cancer using 60282-1-Ig (same clone as 60282-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60282-1-Ig (FAM3C Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60282-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 60282-1-Ig (same clone as 60282-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FAM3C antibody (60282-1-Ig, Clone: 2E10D5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 60282-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

60282-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA FAM3C und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: FAM3C fusion protein Ag5516
• Vollständiger Name: family with sequence similarity 3, member C
• Berechnetes Molekulargewicht: 25 kDa
• Beobachtetes Molekulargewicht: 25 kDa
• GenBank-Zugangsnummer: BC046932
• Gene symbol: FAM3C
• Gene ID (NCBI): 10447
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FAM3C, also known as ILEI, is a secreted protein belonging to the cytokine-like protein family. FAM3C is a signaling cytokine with a GG domain, hydrophobic leader sequence and an N-terminal signal peptide. A change in expression of this protein has been noted in pancreatic cancer-derived cells. FAM3C has been involved in the epithelial to mesenchymal transition, and retinal laminar formation processes in vertebrates. Also, FAM3C may play an important role in inner ear development and thus be involved in cell differentiation and proliferation during inner ear embryogenesis.