FAM65B Polyklonaler Antikörper

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• KD/KO Validated

FAM65B Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 17015-1-AP

Synonyme

C6orf32, DIFF40, DIFF48, FAM65B, KIAA0386, PL48, Protein FAM65B, RIPOR2

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Galerie der Validierungsdaten

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  WB analysis of human skeletal muscle using 17015-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17015-1-AP (FAM65B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human skeletal muscle using 17015-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17015-1-AP (FAM65B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Skelettmuskelgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below

Produktinformation

17015-1-AP bindet in WB, IF, ELISA FAM65B und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FAM65B fusion protein Ag9991
• Vollständiger Name: family with sequence similarity 65, member B
• Berechnetes Molekulargewicht: 118 kDa
• Beobachtetes Molekulargewicht: 115 kDa, 66 kDa
• GenBank-Zugangsnummer: BC001232
• Gene symbol: FAM65B
• Gene ID (NCBI): 9750
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FAM65B is also named as C6orf32, DIFF48, KIAA0386, PL48 and belongs to the FAM65 family.It has 2 isoforms produced by alternative splicing.Isoform 2 play a role in promoting myogenic cell differentiation, cytoskeletal rearrangement and filopodia formation(PMID:17150207). FAM65B is expressed in mononuclear cells, not in myotubes, upon induction of differentiation and cellular fusion, and expression is upregulated during human myoblast differentiation at both the mRNA and protein levels(PMID:17150207).This antibody is specific to the isoform of 119 kDa of FAM65B.

Publikationen

Species	Application	Title
mouse, rat	WB,IF	Proc Natl Acad Sci U S A; FAM65B is a membrane-associated protein of hair cell stereocilia required for hearing.; Authors - Oscar Diaz-Horta
mouse	WB	J Cell Sci; Front Signal-Dependent Accumulation of RHOA Inhibitor FAM65B at Leading Edges Polarizes Neutrophils.; Authors - Kun Gao ; KO Validated
human	WB	FASEB J; Fam65b is important for formation of the HDAC6-dysferlin protein complex during myogenic cell differentiation.; Authors - Anuradha Balasubramanian