FAM76B Polyklonaler Antikörper

FAM76B Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 32882-1-AP

Synonyme

Protein FAM76B

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  WB analysis using 32882-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32882-1-AP (FAM76B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 32882-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 32882-1-AP (FAM76B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 32882-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 32882-1-AP (FAM76B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: J774A.1 cells, RAW 264.7-Zellen, SH-SY5Y-Zellen, U-937-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

32882-1-AP bindet in WB, ELISA FAM76B und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FAM76B fusion protein Ag36658
• Vollständiger Name: family with sequence similarity 76, member B
• Berechnetes Molekulargewicht: 39kDa，339aa
• Beobachtetes Molekulargewicht: 36-39 kDa
• GenBank-Zugangsnummer: NM_144664.4
• Gene symbol: FAM76B
• Gene ID (NCBI): 143684
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FAM76B is a nuclear protein with a molecular weight of about 39 kDa. Its amino acid sequence is highly conserved, and it is highly similar to FAM76B sequence of rats, mice and zebrafish. The protein contains histidine repeat sequence (poly-His domain) and ubiquitination site of Lys-225. FAM76B inhibits the inflammatory reaction mediated by NF-κB by affecting the cytoplasmic translocation of hnRNPA2B1. The expression of inflammatory factors (such as IL-6) increased significantly in FAM76B gene knockout mice, indicating that FAM76B has anti-inflammatory effect.