FAM78A Polyklonaler Antikörper

FAM78A Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 21038-1-AP

Synonyme

Protein FAM78A, C9orf59

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  WB analysis of mouse liver using 21038-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 21038-1-AP (FAM78A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 21038-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 21038-1-AP (FAM78A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 21038-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 21038-1-AP (FAM78A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 21038-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21038-1-AP (FAM78A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 21038-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 21038-1-AP (FAM78A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 21038-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 21038-1-AP (FAM78A Antibody) at dilution of 1:50.

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  IHC staining of human tonsillitis using 21038-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 21038-1-AP (FAM78A Antibody) at dilution of 1:50.

• 

  IHC staining of human colon cancer using 21038-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 21038-1-AP (FAM78A Antibody) at dilution of 1:50.

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  IHC staining of human colon cancer using 21038-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 21038-1-AP (FAM78A Antibody) at dilution of 1:50.

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  IF Staining of HepG2 using 21038-1-AP

  Immunofluorescent analysis of HepG2 cells using 21038-1-AP (FAM78A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, HepG2-Zellen, humanes Lebergewebe, Mausmilzgewebe
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

21038-1-AP bindet in WB, IHC, IF/ICC, ELISA FAM78A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FAM78A fusion protein Ag15366
• Vollständiger Name: family with sequence similarity 78, member A
• Berechnetes Molekulargewicht: 283 aa, 32 kDa
• Beobachtetes Molekulargewicht: 32-35 kDa
• GenBank-Zugangsnummer: BC049388
• Gene symbol: FAM78A
• Gene ID (NCBI): 286336
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.