FAM92B/CIBAR2 Polyklonaler Antikörper

FAM92B/CIBAR2 Polyklonal Antikörper für WB, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Rezensionen (1)

Kat-Nr. : 26413-1-AP

Synonyme

FAM92B, Protein FAM92B, CIBAR2, CBY1-interacting BAR domain-containing protein 2

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• 

  WB analysis of HeLa using 26413-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26413-1-AP (FAM92B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 26413-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 26413-1-AP (FAM92B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse small intestine using 26413-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 26413-1-AP (FAM92B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse lung using 26413-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 26413-1-AP ( FAM92B/CIBAR2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse lung using 26413-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse lung tissue using FAM92B antibody (26413-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse lung using 26413-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse lung tissue using FAM92B antibody (26413-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, COLO 320-Zellen, Maus-Dünndarmgewebe
• Erfolgreiche Detektion in IF-P: Mauslungengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

26413-1-AP bindet in WB, IF-P, ELISA FAM92B/CIBAR2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FAM92B/CIBAR2 fusion protein Ag24802
• Vollständiger Name: family with sequence similarity 92, member B
• Berechnetes Molekulargewicht: 304 aa, 35 kDa
• Beobachtetes Molekulargewicht: 32-35 kDa
• GenBank-Zugangsnummer: BC111944
• Gene symbol: FAM92B
• Gene ID (NCBI): 339145
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FAM92B (family with sequence similarity 92, member B, also named CIBAR2) is a transmembrane protein that plays a crucial role in various cellular processes, including membrane trafficking and cell adhesion. The protein is predicted to be intracellular and is involved in ciliogenesis, likely through the recruitment and fusion of endosomal vesicles at distal appendages during early stages of ciliogenesis.

Publikationen

Species	Application	Title
mouse	IF	Life Sci Alliance; ciBAR1 loss in mice causes laterality defects, pancreatic degeneration, and altered glucose tolerance; Authors - Eunice N Kim

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Eunice (Verified Customer) (10-24-2022)

This was tested on mouse tracheal epithelial cells fixed in 4% PFA overnight and on MEFs fixed for 10 min in ice-cold methanol acetone. Works well.

• Applications: Immunofluorescence, Cell culture
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Tracheal epithelial cells, mouse embryonic fibroblasts (MEFs)