FAM96B Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

FAM96B Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20108-1-PBS

Synonyme

---

• 

  WB analysis using 20108-1-AP (same clone as 20108-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20108-1-AP (FAM96B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20108-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 20108-1-AP (same clone as 20108-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20108-1-AP (FAM96B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20108-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Ramos using 20108-1-AP (same clone as 20108-1-PBS)

  Ramos cells were subjected to SDS PAGE followed by western blot with 20108-1-AP (FAM96B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20108-1-PBS in a different storage buffer formulation.

• 

  IP experiment of K-562 using 20108-1-AP (same clone as 20108-1-PBS)

  IP result of anti-FAM96B (IP:20108-1-AP, 4ug; Detection:20108-1-AP 1:300) with K-562 cells lysate 2400 ug. This data was developed using the same antibody clone with 20108-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 20108-1-AP (same clone as 20108-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20108-1-AP (FAM96B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20108-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 20108-1-AP (same clone as 20108-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20108-1-AP (FAM96B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20108-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 20108-1-AP (same clone as 20108-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20108-1-AP (FAM96B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20108-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 20108-1-AP (same clone as 20108-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20108-1-AP (FAM96B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20108-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

20108-1-PBS bindet in WB, IHC, IP, Indirect ELISA FAM96B und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FAM96B fusion protein Ag13827
• Vollständiger Name: family with sequence similarity 96, member B
• Berechnetes Molekulargewicht: 163 aa, 18 kDa
• Beobachtetes Molekulargewicht: 18 kDa
• GenBank-Zugangsnummer: BC001733
• Gene symbol: FAM96B
• Gene ID (NCBI): 51647
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FAM96B, also known as MIP18 or CIAO2B (Cytosolic iron-sulfur assembly component 2B), is a component of the cytosolic iron-sulfur protein assembly (CIA) complex involved in iron homeostasis and part of the MMXD complex contributed to proper chromosome segregation (PMID: 36503010). FAM96B may act as a scaffold protein to recruit CKB and the other components of the MMXD complex to associate with the spindle and mitotic apparatus and contributes to chromosome segregation.