FANCA Polyklonaler Antikörper

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• KD/KO Validated

FANCA Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(8)

Kat-Nr. : 11975-1-AP

Synonyme

FA, FA1, FAA, FACA, FAH

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  WB analysis of HeLa cells using 11975-1-AP

  WB results of FANCA (11975-1-AP, 1:500) with si-control and si-FANCA transfected HeLa cells.

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  WB analysis using 11975-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11975-1-AP (FANCA antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 11975-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11975-1-AP (FANCA Antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human colon cancer using 11975-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11975-1-AP (FANCA Antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human stomach cancer using 11975-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11975-1-AP (FANCA antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 11975-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 11975-1-AP (FANCA antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse intestine using 11975-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse intestine tissue slide using 11975-1-AP (FANCA antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse thymus using 11975-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 11975-1-AP (FANCA antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat small intestine using 11975-1-AP

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 11975-1-AP (FANCA antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 11975-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 11975-1-AP (FANCA antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COLO 320-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Zervixkarzinomgewebe, humanes Magenkrebsgewebe, Maus-Thymusgewebe, Ratten-Dünndarmgewebe, Rattenhodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

11975-1-AP bindet in WB, IHC, IF, IP, ELISA FANCA und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FANCA fusion protein Ag2598
• Vollständiger Name: Fanconi anemia, complementation group A
• Berechnetes Molekulargewicht: 163 kDa
• Beobachtetes Molekulargewicht: 163 kDa
• GenBank-Zugangsnummer: BC008979
• Gene symbol: FANCA
• Gene ID (NCBI): 2175
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FANCA, also named as FAA, FACA and FANCH, is a DNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. It's involved in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability.

Publikationen

Species	Application	Title
	WB	Nat Struct Mol Biol; Fanconi anemia proteins participate in a break-induced-replication-like pathway to counter replication stress.; Authors - Xinlin Xu ; KO Validated
mouse	WB	EMBO Mol Med; WNT inhibition creates a BRCA-like state in Wnt-addicted cancer.; Authors - Amanpreet Kaur
human	WB	Cell Rep; C1orf112 teams up with FIGNL1 to facilitate RAD51 filament disassembly and DNA interstrand cross-link repair; Authors - Zenan Zhou
human	WB,IHC	Acta Biochim Biophys Sin (Shanghai); FANCA facilitates G1/S cell cycle advancement, proliferation, migration and invasion in gastric cancer; Authors - Wei Wang ; KD Validated
human	WB	Gene; Circular RNA Circ_0000119 promotes gastric cancer progression via circ_0000119/miR-502-5p/MTBP axis; Authors - Mengyan Xie
human	IP,IHC,IF	Gene; Identification of rare heterozygous missense mutations in FANCA in esophageal atresia patients using next-generation sequencing.; Authors - Yu Feng