FANCG Polyklonaler Antikörper

FANCG Polyklonal Antikörper für IF, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 10215-1-AP

Synonyme

DNA repair protein XRCC9, FAG, FANCG, Fanconi anemia group G protein, Protein FACG, XRCC9

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 10215-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10215-1-AP (FANCG antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 10215-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 10215-1-AP (FANCG antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10215-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10215-1-AP (FANCG antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 10215-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10215-1-AP (FANCG antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 10215-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10215-1-AP (FANCG antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 10215-1-AP

  IP Result of anti-FANCG (IP:10215-1-AP, 4ug; Detection:10215-1-AP 1:300) with mouse testis tissue lysate 4000ug.

• 

  IF Staining of HeLa using 10215-1-AP

  Immunofluorescent analysis of HeLa cells using 10215-1-AP (FANCG antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HeLa-Zellen, K-562-Zellen, Maushodengewebe, Maus-Thymusgewebe
• Erfolgreiche IP: Maushodengewebe
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 1 publications below

Produktinformation

10215-1-AP bindet in WB, IP, IF, ELISA FANCG und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FANCG fusion protein Ag0249
• Vollständiger Name: Fanconi anemia, complementation group G
• Berechnetes Molekulargewicht: 69 kDa
• Beobachtetes Molekulargewicht: 65-68 kDa
• GenBank-Zugangsnummer: BC000032
• Gene symbol: FANCG
• Gene ID (NCBI): 2189
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FANCG, also named as XRCC9, is a vDNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. It's a candidate tumor suppressor gene. FANCA and FANCG are critical for the localization of the FANC core complex. FANCA and FANCG form a stable complex with a molecular weight of ∼500 kDa that is predicted to be a heterotetramer (PMID: 32002546).

Publikationen

Species	Application	Title
human	WB	Sci Adv; Deficiency in mammalian STN1 promotes colon cancer development via inhibiting DNA repair; Authors - Dinh Duc Nguyen
human	IF	J Cancer; Significant value of XRCC2 and XRCC9 expression in the prognosis of human ovarian carcinoma.; Authors - Yi Liu
human	WB	Clin Transl Oncol; Therapeutic benefits of niraparib tosylate as radio sensitizer in esophageal squamous cell carcinoma: an in vivo and in vitro preclinical study.; Authors - Yuzhong Cui