FANCL Polyklonaler Antikörper

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• KD/KO Validated

FANCL Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14454-1-AP

Synonyme

FAAP43, FANCL, Fanconi anemia group L protein, PHF9, POG

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  WB analysis of A549 using 14454-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 14454-1-AP (FANCL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 14454-1-AP

  WB result of FANCL antibody (14454-1-AP; 1:200; incubated at room temperature for 1.5 hours) with sh-Control and sh-FANCL transfected HEK-293 cells.

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  IHC staining of human lung cancer using 14454-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14454-1-AP (FANCL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 14454-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14454-1-AP (FANCL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 14454-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14454-1-AP (FANCL Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 14454-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14454-1-AP (FANCL Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HEK-293-Zellen, HEK-Zellen, HeLa-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

14454-1-AP bindet in WB, IHC, ELISA FANCL und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FANCL fusion protein Ag6031
• Vollständiger Name: Fanconi anemia, complementation group L
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 28 kDa
• GenBank-Zugangsnummer: BC054517
• Gene symbol: FANCL
• Gene ID (NCBI): 55120
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fanconi anemia (FA) is a hereditary bone marrow failure syndrome associated with developmental defects and cancer predisposition. The FA nuclear core complex consists of FANCA, B, C, E, F, G, L, and M. FANCL is a RING-type E3 ubiquitin ligase that monoubiquitinates FANCD2 and FANCI, a key functional role facilitated by other members of the nuclear core complex (PMID: 23783032). FANCL plays an important role in the repair of cisplatin-induced DNA damage (PMID: 26385482) and participates directly in support of stem cell function (PMID: 23783032). FANCL can be detected as 40-43 kDa (full-length) and ~28 kDa.