FANCL Polyklonaler Antikörper

FANCL Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 19187-1-AP

Synonyme

PHF9, Fanconi anemia-associated polypeptide of 43 kDa, Fanconi anemia group L protein, FAAP43, EC:2.3.2.27

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  WB analysis of human brain using 19187-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 19187-1-AP (FANCL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 19187-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19187-1-AP (FANCL antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 19187-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 19187-1-AP (FANCL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 19187-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 19187-1-AP (FANCL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 19187-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 19187-1-AP (FANCL antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe, HEK-293-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

19187-1-AP bindet in WB, IF/ICC, ELISA FANCL und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FANCL fusion protein Ag6192
• Vollständiger Name: Fanconi anemia, complementation group L
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 41-43 kDa
• GenBank-Zugangsnummer: BC054517
• Gene symbol: FANCL
• Gene ID (NCBI): 55120
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fanconi anemia (FA) is a hereditary bone marrow failure syndrome associated with developmental defects and cancer predisposition. The FA nuclear core complex consists of FANCA, B, C, E, F, G, L, and M. FANCL is a RING-type E3 ubiquitin ligase that monoubiquitinates FANCD2 and FANCI, a key functional role facilitated by other members of the nuclear core complex (PMID: 23783032). FANCL plays an important role in the repair of cisplatin-induced DNA damage (PMID: 26385482) and participates directly in support of stem cell function (PMID: 23783032). FANCL can be detected as 40-43 kDa (full-length) and ~28 kDa.