FANCM Polyklonaler Antikörper

FANCM Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 12954-1-AP

Synonyme

KIAA1596, Fanconi anemia-associated polypeptide of 250 kDa, Fanconi anemia group M protein, FAAP250, EC:3.6.4.13

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  WB analysis of HeLa using 12954-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12954-1-AP (FANCM antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below

Produktinformation

12954-1-AP bindet in WB, ELISA FANCM und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FANCM fusion protein Ag3618
• Vollständiger Name: Fanconi anemia, complementation group M
• Berechnetes Molekulargewicht: 2048 aa, 232 kDa
• Beobachtetes Molekulargewicht: 250 kDa, 232 kDa
• GenBank-Zugangsnummer: BC036056
• Gene symbol: FANCM
• Gene ID (NCBI): 57697
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FANCM (Fanconi anemia group M protein) is a DNA-dependent ATPase component of the Fanconi anemia (FA) core complex (PMID: 16116422). It is required for the normal activation of the FA pathway, leading to monoubiquitination of the FANCI-FANCD2 complex in response to DNA damage, cellular resistance to DNA cross-linking drugs, and prevention of chromosomal breakage (PMID:16116422; 19423727; 20347428). FANCM is localized in the cell nucleus and can bind double-stranded DNA (dsDNA), fork-structured DNA (fsDNA), and Holliday junction substrates (PMID: 20347428; 20347429).

Publikationen

Species	Application	Title
mouse	WB	EMBO J; m6 A deposition is regulated by PRMT1-mediated arginine methylation of METTL14 in its disordered C-terminal region.; Authors - Zhihao Wang
human	WB	Onco Targets Ther; The Regulatory Mechanism and Biological Significance of Mitochondrial Calcium Uniporter in the Migration, Invasion, Angiogenesis and Growth of Gastric Cancer.; Authors - Xiaofei Wang
human	WB	mSystems; Dual RNA sequencing of Helicobacter pylori and host cell transcriptomes reveals ontologically distinct host-pathogen interaction; Authors - Wei Hu
mouse	WB	Cell Prolif; Lin28a forms an RNA-binding complex with Igf2bp3 to regulate m6A-modified stress response genes in stress granules of muscle stem cells; Authors - Dan Song