FANCM Rekombinanter Antikörper

FANCM Rekombinant Antikörper für IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

230233G5

Kat-Nr. : 82897-2-PBS

Synonyme

Fanconi anemia-associated polypeptide of 250 kDa, Fanconi anemia group M protein, FAAP250, EC:3.6.4.13, ATP-dependent RNA helicase FANCM

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  IP experiment of HepG2 using 82897-2-RR (same clone as 82897-2-PBS)

  IP result of anti-FANCM (IP:82897-2-RR, 4ug; Detection:82897-2-RR 1:500) with HepG2 cells lysate 400 ug. This data was developed using the same antibody clone with 82897-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 82897-2-RR (same clone as 82897-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82897-2-RR (FANCM antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82897-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 82897-2-RR (same clone as 82897-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82897-2-RR (FANCM antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82897-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 82897-2-RR (same clone as 82897-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FANCM antibody (82897-2-RR, Clone: 230233G5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 82897-2-PBS in a different storage buffer formulation.

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  FC experiment of PC-3 using 82897-2-RR (same clone as 82897-2-PBS)

  1x10^6 PC-3 cells were intracellularly stained with 0.25 ug FANCM Recombinant antibody (82897-2-RR, Clone:230233G5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82897-2-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

82897-2-PBS bindet in IHC, IF/ICC, FC (Intra), IP, Indirect ELISA FANCM und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: FANCM fusion protein Ag34447
• Vollständiger Name: Fanconi anemia, complementation group M
• GenBank-Zugangsnummer: BC140776
• Gene symbol: FANCM
• Gene ID (NCBI): 57697
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.