FARP1 Polyklonaler Antikörper

FARP1 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 24014-1-AP

Synonyme

Pleckstrin homology domain-containing family C member 2, PH domain-containing family C member 2, FERM, RhoGEF and pleckstrin domain-containing protein 1, FERM, ARHGEF and pleckstrin domain-containing protein 1, Chondrocyte-derived ezrin-like protein

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  WB analysis using 24014-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24014-1-AP (FARP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 24014-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 24014-1-AP (FARP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 24014-1-AP

  IP result of anti-FARP1 (IP:24014-1-AP, 4ug; Detection:24014-1-AP 1:1000) with HeLa cells lysate 1200 ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen
• Erfolgreiche IP: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

24014-1-AP bindet in WB, IP, ELISA FARP1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FARP1 fusion protein Ag21225
• Vollständiger Name: FERM, RhoGEF (ARHGEF) and pleckstrin domain protein 1 (chondrocyte-derived)
• Berechnetes Molekulargewicht: 1076 aa, 122 kDa
• Beobachtetes Molekulargewicht: 122 kDa
• GenBank-Zugangsnummer: BC071592
• Gene symbol: FARP1
• Gene ID (NCBI): 10160
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FARP1 is a protein containing a FERM (4.2, exrin, radixin, moesin) domain, a Dbl homology domain, and two pleckstrin homology domains. These domains are found in guanine nucleotide exchange factors and proteins that link the cytoskeleton to the cell membrane. The encoded protein functions in neurons to promote dendritic growth.