FARSB Polyklonaler Antikörper

FARSB Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 16341-1-AP

Synonyme

Phenylalanine--tRNA ligase beta subunit, PheHB, HSPC173, FRSB, FARSLB

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  WB analysis using 16341-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16341-1-AP (FARSB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16341-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16341-1-AP (FARSB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 16341-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16341-1-AP (FARSB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 16341-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16341-1-AP (FARSB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 16341-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 16341-1-AP (FARSB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 16341-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 16341-1-AP (FARSB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 16341-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16341-1-AP (FARSB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 16341-1-AP

  IP result of anti-FARSB (IP:16341-1-AP, 4ug; Detection:16341-1-AP 1:500) with HeLa cells lysate 4000ug.

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  IHC staining of mouse brain using 16341-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16341-1-AP (FARSB antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 16341-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16341-1-AP (FARSB antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 16341-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 16341-1-AP (FARSB antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 16341-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 16341-1-AP (FARSB antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 16341-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 16341-1-AP (FARSB antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 16341-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 16341-1-AP (FARSB antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 16341-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16341-1-AP (FARSB antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 16341-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16341-1-AP (FARSB antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 16341-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 16341-1-AP (FARSB antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 16341-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 16341-1-AP (FARSB antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 16341-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 16341-1-AP (FARSB antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HeLa-Zellen, HL-60-Zellen, humanes Lebergewebe, K-562-Zellen, Maushirngewebe, Mauslebergewebe, PC-3-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Pankreasgewebe, Maus-Cerebellum-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Produktinformation

16341-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA FARSB und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FARSB fusion protein Ag9522
• Vollständiger Name: phenylalanyl-tRNA synthetase, beta subunit
• Berechnetes Molekulargewicht: 589 aa, 66 kDa
• Beobachtetes Molekulargewicht: 66 kDa
• GenBank-Zugangsnummer: BC017783
• Gene symbol: FARSB
• Gene ID (NCBI): 10056
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
		J Am Chem Soc; Oxidation-Controlled, Strain-Promoted Tellurophene-Alkyne Cycloaddition (OSTAC): A Bioorthogonal Tellurophene-Dependent Conjugation Reaction; Authors - Yong Jia Bu
human,rat	WB	Nat Commun; Cis- and trans-resveratrol have opposite effects on histone serine-ADP-ribosylation and tyrosine induced neurodegeneration.; Authors - Megha Jhanji
rat	WB	Neural Regen Res; A novel therapeutic target for peripheral nerve injury-related diseases: aminoacyl-tRNA synthetases.; Authors - Byung Sun Park
human,mouse	WB,IF	Nat Commun; Phenylalanine-tRNA aminoacylation is compromised by ALS/FTD-associated C9orf72 C4G2 repeat RNA; Authors - Mirjana Malnar Črnigoj
human,mouse	WB,IHC,IF	Int J Mol Sci; FARSB Facilitates Hepatocellular Carcinoma Progression by Activating the mTORC1 Signaling Pathway; Authors - Yaofeng Wang