FARSB Monoklonaler Antikörper

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FARSB Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1H10C2

Kat-Nr. : 67924-1-PBS

Synonyme

FARSB, FARSLB, FRSB, HSPC173, PheHB, PheRS

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  WB analysis using 67924-1-Ig (same clone as 67924-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67924-1-Ig (FARSB antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67924-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 67924-1-Ig (same clone as 67924-1-PBS)

  WB result of FARSB antibody (67924-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FARSB transfected HepG2 cells. This data was developed using the same antibody clone with 67924-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 67924-1-Ig (same clone as 67924-1-PBS)

  WB result of FARSB antibody (67924-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FARSB transfected HeLa cells. This data was developed using the same antibody clone with 67924-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 67924-1-Ig (same clone as 67924-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67924-1-Ig (FARSB antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67924-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67924-1-Ig (same clone as 67924-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67924-1-Ig (FARSB antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67924-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67924-1-Ig (same clone as 67924-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67924-1-Ig (FARSB antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67924-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 67924-1-Ig (same clone as 67924-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67924-1-Ig (FARSB antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67924-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 67924-1-Ig (same clone as 67924-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67924-1-Ig (FARSB antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67924-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 67924-1-Ig (same clone as 67924-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67924-1-Ig (FARSB antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67924-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 67924-1-Ig (same clone as 67924-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67924-1-Ig (FARSB antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67924-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 67924-1-Ig (same clone as 67924-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67924-1-Ig (FARSB antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67924-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 67924-1-Ig (same clone as 67924-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67924-1-Ig (FARSB antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67924-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 67924-1-Ig (same clone as 67924-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using FARSB antibody (67924-1-Ig, Clone: 1H10C2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67924-1-PBS in a different storage buffer formulation.

• 

  IF Staining of U-251 using 67924-1-Ig (same clone as 67924-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed U-251 cells using FARSB antibody (67924-1-Ig, Clone: 1H10C2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67924-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67924-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA FARSB und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: FARSB fusion protein Ag9695
• Vollständiger Name: phenylalanyl-tRNA synthetase, beta subunit
• Berechnetes Molekulargewicht: 589 aa, 66 kDa
• Beobachtetes Molekulargewicht: 66 kDa
• GenBank-Zugangsnummer: BC017783
• Gene symbol: FARSB
• Gene ID (NCBI): 10056
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.