Fas/CD95 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

Fas/CD95 Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(15)

CloneNo.

1G7F7

Kat-Nr. : 60196-1-Ig

Synonyme

FAS, 1G7F7, ALPS1A, APO-1, Apo-1 antigen

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  WB analysis of Jurkat using 60196-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60196-1-Ig (FAS/CD95 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 60196-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 60196-1-Ig (FAS/CD95 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 60196-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 60196-1-Ig (FAS/CD95 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-937 using 60196-1-Ig

  U-937 cells were subjected to SDS PAGE followed by western blot with 60196-1-Ig (FAS/CD95 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 60196-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60196-1-Ig (FAS/CD95 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 60196-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60196-1-Ig (FAS/CD95 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 60196-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60196-1-Ig (FAS/CD95 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 60196-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60196-1-Ig (FAS/CD95 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HeLa-Zellen, K-562-Zellen, U-937-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 13 publications below
• IHC: See 4 publications below
• IF: See 2 publications below

Produktinformation

60196-1-Ig bindet in WB, IHC, IF, ELISA Fas/CD95 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Zebrafisch
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Fas/CD95 fusion protein Ag3718
• Vollständiger Name: Fas (TNF receptor superfamily, member 6)
• Berechnetes Molekulargewicht: 35-38 kDa
• Beobachtetes Molekulargewicht: 38-45 kDa
• GenBank-Zugangsnummer: BC012479
• Gene symbol: Fas/CD95
• Gene ID (NCBI): 355
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FAS, also named as CD95, APO-1, APT1, FAS1 and TNFRSF6, is a receptor for TNFSF6/FASLG. It is a cell surface receptor belonging to the TNF receptor superfamily, can mediate apoptosis by ligation with an agonistic anti-Fas antibody or Fas ligand. Stimulation of Fas results in the aggregation of its intracellular death domains, leading to the formation of the death-inducing signaling complex (DISC). FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro). This anti-Fas monoclonal antibody can be used to induce apoptosis in cell cultures through Fas by imitating the Fas-ligand.

Publikationen

Species	Application	Title
human	WB	Cell; Neutrophil elastase selectively kills cancer cells and attenuates tumorigenesis.; Authors - Chang Cui ; KO Validated
human	WB,IF	Biochim Biophys Acta; Down-regulation of islet amyloid polypeptide expression induces death of human annulus fibrosus cells via mitochondrial and death receptor pathways.; Authors - Xinghuo Wu
	WB,IHC,IF	Mol Neurobiol; Ox-LDL Induces Neuron Apoptosis and Worsens Neurological Outcomes in aSAH via Fas/FADD Pathway; Authors - Yabin Song
	WB	J Physiol Biochem; miR-128-3p regulates 3T3-L1 adipogenesis and lipolysis by targeting Pparg and Sertad2.; Authors - Chen Chen
	WB	iScience; Loss of Adipose Growth Hormone Receptor in Mice Enhances Local Fatty Acid Trapping and Impairs Brown Adipose Tissue Thermogenesis.; Authors - Liyuan Ran
Zebrafish	IHC	Chemosphere; Possible involvement of Fas/FasL-dependent apoptotic pathway in α-bisabolol induced cardiotoxicity in zebrafish embryos.; Authors - Meng Jin