Fas/CD95 Monoklonaler Antikörper

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• KD/KO Validated

Fas/CD95 Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1G7F7

Kat-Nr. : 60196-1-PBS

Synonyme

FAS, 1G7F7, ALPS1A, APO-1, Apo-1 antigen

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• 

  WB analysis of Jurkat using 60196-1-Ig (same clone as 60196-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60196-1-Ig (FAS/CD95 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60196-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 60196-1-Ig (same clone as 60196-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 60196-1-Ig (FAS/CD95 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60196-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 60196-1-Ig (same clone as 60196-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 60196-1-Ig (FAS/CD95 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60196-1-PBS in a different storage buffer formulation.

• 

  WB analysis of U-937 using 60196-1-Ig (same clone as 60196-1-PBS)

  U-937 cells were subjected to SDS PAGE followed by western blot with 60196-1-Ig (FAS/CD95 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60196-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 60196-1-Ig (same clone as 60196-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60196-1-Ig (FAS/CD95 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60196-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 60196-1-Ig (same clone as 60196-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60196-1-Ig (FAS/CD95 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60196-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 60196-1-Ig (same clone as 60196-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60196-1-Ig (FAS/CD95 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60196-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 60196-1-Ig (same clone as 60196-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60196-1-Ig (FAS/CD95 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60196-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

60196-1-PBS bindet in WB, IHC, ELISA Fas/CD95 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Fas/CD95 fusion protein Ag3718
• Vollständiger Name: Fas (TNF receptor superfamily, member 6)
• Berechnetes Molekulargewicht: 35-38 kDa
• Beobachtetes Molekulargewicht: 38-45 kDa
• GenBank-Zugangsnummer: BC012479
• Gene symbol: Fas/CD95
• Gene ID (NCBI): 355
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FAS, also named as CD95, APO-1, APT1, FAS1 and TNFRSF6, is a receptor for TNFSF6/FASLG. It is a cell surface receptor belonging to the TNF receptor superfamily, can mediate apoptosis by ligation with an agonistic anti-Fas antibody or Fas ligand. Stimulation of Fas results in the aggregation of its intracellular death domains, leading to the formation of the death-inducing signaling complex (DISC). FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro). This anti-Fas monoclonal antibody can be used to induce apoptosis in cell cultures through Fas by imitating the Fas-ligand.