Fas/CD95 Rekombinanter Antikörper

Fas/CD95 Rekombinant Antikörper für IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

240340E11

Kat-Nr. : 83400-5-RR

Synonyme

FAS, APT1, Apoptosis-mediating surface antigen FAS, Apo-1 antigen, APO-1

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  IF Staining of Raji using 83400-5-RR

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using Fas/CD95 antibody (83400-5-RR, Clone: 240340E11 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

• 

  IF Staining of Raji using 83400-5-RR

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using Fas/CD95 antibody (83400-5-RR, Clone: 240340E11 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

• 

  IF Staining of Jurkat using 83400-5-RR

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using Fas/CD95 antibody (83400-5-RR, Clone: 240340E11 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

• 

  IF Staining of Jurkat using 83400-5-RR

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using Fas/CD95 antibody (83400-5-RR, Clone: 240340E11 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

• 

  Affinity and Kinetic Characterization of 83400-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83400-5-RR against Human Fas/CD95 were performed. The affinity constant is 0.927 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: Raji-Zellen, Jurkat-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83400-5-RR bindet in IF/ICC, ELISA Fas/CD95 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Fusionsprotein
• Vollständiger Name: Fas (TNF receptor superfamily, member 6)
• Berechnetes Molekulargewicht: 38 kDa
• GenBank-Zugangsnummer: NM_000043.6
• Gene symbol: Fas/CD95
• Gene ID (NCBI): 355
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fas (CD95/APO-1) is a transmembrane glycoprotein belonging to the tumor necrosis factor (TNF) receptor superfamily. It can mediate apoptosis by ligation with an agonistic anti-Fas antibody or Fas ligand. Stimulation of Fas results in the aggregation of its intracellular death domains, leading to the formation of the death-inducing signaling complex (DISC). FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both.