FASN Monoklonaler Antikörper

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FASN Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1B6E4

Kat-Nr. : 66591-1-PBS

Synonyme

Type I fatty acid synthase, fatty acid synthase, FAS, 1B6E4

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  WB analysis of HeLa using 66591-1-Ig (same clone as 66591-1-PBS)

  WB result of FASN antibody (66591-1-Ig; 1:800; incubated at room temperature for 1.5 hours) with sh-Control and sh-FASN transfected HeLa cells. This data was developed using the same antibody clone with 66591-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 66591-1-Ig (same clone as 66591-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66591-1-Ig (FASN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66591-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66591-1-Ig (same clone as 66591-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66591-1-Ig (FASN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66591-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 66591-1-Ig (same clone as 66591-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66591-1-Ig (FASN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66591-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66591-1-Ig (same clone as 66591-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66591-1-Ig (FASN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66591-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66591-1-Ig (same clone as 66591-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66591-1-Ig (FASN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66591-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66591-1-Ig (same clone as 66591-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using FASN antibody (66591-1-Ig, Clone: 1B6E4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66591-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66591-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA FASN und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: FASN fusion protein Ag0975
• Vollständiger Name: fatty acid synthase
• Berechnetes Molekulargewicht: 272 kDa
• Beobachtetes Molekulargewicht: 272 kDa
• GenBank-Zugangsnummer: BC007909
• Gene symbol: FASN
• Gene ID (NCBI): 2194
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FASN gene codes for an enzyme essential for de novo fatty acid synthesis and cellular substrate energy metabolism. Active FASN is a homodimer in which each peptide subunit has a molecular weight of 260 kDa. FASN is overexpressed in various types of cancer including glioblastomas and is a potential therapeutic target. Recently FASN has been reported to contribute to the neurogenesis since FASN mutation caused intellectual disability in mice.