FASN Rekombinanter Antikörper

FASN Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1L9

Kat-Nr. : 81079-1-RR

Synonyme

3-oxoacyl-[acyl-carrier-protein] synthase, 3-oxoacyl-[acyl-carrier-protein] reductase, 3-hydroxyacyl-[acyl-carrier-protein] dehydratase, 1L9, [Acyl-carrier-protein] S-malonyltransferase

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  WB analysis using 81079-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 81079-1-RR (FASN antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 81079-1-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using FASN antibody (81079-1-RR, Clone: 1L9 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  Affinity and Kinetic Characterization of 81079-1-RR

  Biolayer interferometry (BLl) kinetic assays of 81079-1-RR against Human FASN were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, MCF-7-Zellen, Mauslebergewebe, Neuro-2a-Zellen, Rattenlebergewebe, U-251-Zellen
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

81079-1-RR bindet in WB, IF/ICC, ELISA FASN und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: FASN fusion protein Ag0975
• Vollständiger Name: fatty acid synthase
• Berechnetes Molekulargewicht: 272 kDa
• Beobachtetes Molekulargewicht: 250-270 kDa
• GenBank-Zugangsnummer: BC007909
• Gene symbol: FASN
• Gene ID (NCBI): 2194
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FASN gene codes for an enzyme essential for de novo fatty acid synthesis and cellular substrate energy metabolism. Active FASN is a homodimer in which each peptide subunit has a molecular weight of 260 kDa. FASN is overexpressed in various types of cancer including glioblastomas and is a potential therapeutic target. Recently FASN has been reported to contribute to the neurogenesis since FASN mutation caused intellectual disability in mice.