FASTKD2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

FASTKD2 Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, RIP, ELISA

Konjugation

Unkonjugiert

Publikationen(22)

Rezensionen (1)

Kat-Nr. : 17464-1-AP

Synonyme

FASTKD 2, FAST kinase domain-containing protein 2, mitochondrial

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  WB analysis using 17464-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17464-1-AP (FASTKD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 17464-1-AP

  WB result of FASTKD2 antibody (17464-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FASTKD2 transfected HepG2 cells.

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  IP experiment of HepG2 using 17464-1-AP

  IP result of anti-FASTKD2 (IP:17464-1-AP, 4ug; Detection:17464-1-AP 1:1000) with HepG2 cells lysate 2040 ug.

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  IF Staining of COS7 cells using 17464-1-AP

  IF result of anti-FASTKD2 (17464-1-AP) with COS7 cells.

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  IF Staining of U2OS using 17464-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using FASTKD2 antibody (17464-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of COS7 cells using 17464-1-AP

  IF result of anti- FASTKD2 (17464-1-AP) with FASTKD2-YFP overexpression in COS7 cells.

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  IF Staining of U2OS cells using 17464-1-AP

  IF result of anti-FASTKD2 (17464-1-AP) with U2OS cells.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, K-562-Zellen
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IF/ICC: COS7-Zellen, U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 13 publications below
• IHC: See 1 publications below
• IF: See 12 publications below
• RIP: See 1 publications below

Produktinformation

17464-1-AP bindet in WB, IHC, IF/ICC, IP, RIP, ELISA FASTKD2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FASTKD2 fusion protein Ag11314
• Vollständiger Name: FAST kinase domains 2
• Berechnetes Molekulargewicht: 710 aa, 81 kDa
• Beobachtetes Molekulargewicht: 55-65 kDa
• GenBank-Zugangsnummer: BC001544
• Gene symbol: FASTKD2
• Gene ID (NCBI): 22868
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FASTKD2 encodes a FAS-activated serine-threonine (FAST) kinase domain 2 which locates in the mitochondrial inner compartment. FASTKD2 is a member of the human FASTK protein family, formed by six architecturally related proteins named FASTK and FASTKD1-5 that localize to mitochondria. The predicted MW of FASTKD2 is 81 kDa, yet the western blot of FASTKD2 revealed a band of ~60 kDa. (PMID: 26370583, PMID: 26990993, PMID: 25683715)

Publikationen

Species	Application	Title
human	IF,WB	Nat Cell Biol; Mitochondrial RNA granules are fluid condensates positioned by membrane dynamics.; Authors - Timo Rey
human	WB	Cell Metab; A Genome-wide CRISPR Death Screen Identifies Genes Essential for Oxidative Phosphorylation.; Authors - Jason D Arroyo
human	IF	Proc Natl Acad Sci U S A; Cytosolic N6AMT1-dependent translation supports mitochondrial RNA processing; Authors - Mads M Foged
human	IF	Nucleic Acids Res; FASTKD1 and FASTKD4 have opposite effects on expression of specific mitochondrial RNAs, depending upon their endonuclease-like RAP domain.; Authors - Erik Boehm
human	WB	EMBO Rep; A pseudouridine synthase module is essential for mitochondrial protein synthesis and cell viability.; Authors - Hana Antonicka
human	WB, IF, RIP	Cell Rep; Mitochondrial RNA Granules Are Centers for Posttranscriptional RNA Processing and Ribosome Biogenesis.; Authors - Hana Antonicka ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Erik (Verified Customer) (02-25-2021)

We used the antibody at 1:500 dilution, and it produced two clean bands for the two protein isoforms annotated in UniProt, at the expected height

• Applications: Western Blot
• Cell Tissue Type: PANC1 and MIAPACA2 cell lines

FASTKD2 Antibody Western Blot validation ( dilution) in PANC1 and MIAPACA2 cell lines (Cat no:17464-1-AP)