FAT1 Rekombinanter Antikörper

FAT1 Rekombinant Antikörper für WB, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241602F9

Kat-Nr. : 84279-3-PBS

Synonyme

241602F9, CDHF7, FAT, hFat1, ME5

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  WB analysis of HeLa using 84279-3-RR (same clone as 84279-3-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 84279-3-RR (FAT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84279-3-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP01192-3

  Cytometric bead array standard curve of MP01192-3, FAT1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84279-4-PBS. Detection antibody: 84279-3-PBS. Standard: Ag33957. Range: 0.625-80 ng/mL.

• 

  Affinity and Kinetic Characterization of 84279-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84279-3-RR against Human FAT1 were performed. The affinity constant is 0.375 nM.

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Geprüfte Anwendungen

Produktinformation

84279-3-PBS bindet in WB, Cytometric bead array, Indirect ELISA FAT1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: FAT1 fusion protein Ag33957
• Vollständiger Name: FAT tumor suppressor homolog 1 (Drosophila)
• Beobachtetes Molekulargewicht: 506-600 kDa, 85 kDa
• Gene symbol: FAT1
• Gene ID (NCBI): 2195
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FAT1, also known as hFat1, belongs to a member of the cadherin superfamily, has been proposed to play roles in cerebral development, glomerular slit formation, and also to act as a tumor suppressor, but its mechanisms of action have not been elucidated. It is expected to be located in cell membrane and nucleus, which is expressed in many epithelial and some endothelial and smooth muscle cells. The calculated molecular weight of FAT1 is 506 kDa and there is glycosylation modification of the protein. To examine functions of the transmembrane and cytoplasmic domains, they were expressed in HEK-293 and HeLa cells as chimeric proteins in fusion with EGFP and extracellular domains derived from E-cadherin. Proteins comprising the transmembrane domain localized to the membrane fraction (PMID: 15922730, 26373379). The high molecular mass band observed at the expected 500-kDa size of FAT1 is indicated by an arrow together with a prominent band at 85 kDa (PMID: 21680732).