FATP2 Monoklonaler Antikörper

FATP2 Monoklonal Antikörper für WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Ratte

Anwendung

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2C10D7

Kat-Nr. : 68074-1-PBS

Synonyme

SLC27A2, EC:6.2.1.24, EC:6.2.1.15, EC:6.2.1.-, Arachidonate--CoA ligase

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  WB analysis using 68074-1-Ig (same clone as 68074-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68074-1-Ig (SLC27A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68074-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 68074-1-Ig (same clone as 68074-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 68074-1-Ig (SLC27A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68074-1-PBS in a different storage buffer formulation.

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  WB analysis of rat liver using 68074-1-Ig (same clone as 68074-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 68074-1-Ig (FATP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68074-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 68074-1-Ig (same clone as 68074-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68074-1-Ig (FATP2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68074-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 68074-1-Ig (same clone as 68074-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68074-1-Ig (FATP2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68074-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 68074-1-Ig (same clone as 68074-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FATP2 antibody (68074-1-Ig, Clone: 2C10D7 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 68074-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50832-1

  Cytometric bead array standard curve of MP50832-1, SLC27A2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68074-1-PBS. Detection antibody: 68074-2-PBS. Standard:Ag5390. Range: 12.5-200 ng/mL.

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Geprüfte Anwendungen

Produktinformation

68074-1-PBS bindet in WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA FATP2 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: FATP2 fusion protein Ag5390
• Vollständiger Name: solute carrier family 27 (fatty acid transporter), member 2
• Berechnetes Molekulargewicht: 567 aa, 65 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC057770
• Gene symbol: FATP2/SLC27A2
• Gene ID (NCBI): 11001
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FATP2 is a member of the FATP family which functions in lipid and bile metabolism. It is a 70-kDa protein predominantly expressed in liver and kidney. Kidney FATP2 is localized exclusively to proximal tubule epithelial cells along the apical but not the basolateral membrane, and regulates lipoapoptosis. FATP2 is involved in metabolism-related diseases including nonalcoholic fatty liver disease (NAFLD) and type 2 diabetes mellitus (T2DM), and is a potential clinical biomarker and therapeutic target.