FATP5/SLC27A5 Polyklonaler Antikörper

FATP5/SLC27A5 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 25347-1-AP

Synonyme

FATP5, SLC27A5, Cholate--CoA ligase, Bile acyl-CoA synthetase, Bile acid-CoA ligase

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  WB analysis using 25347-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25347-1-AP (FATP5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of rat liver using 25347-1-AP

  IP result of anti-FATP5 (IP:25347-1-AP, 4ug; Detection:25347-1-AP 1:5000) with rat liver tissue lysate 2695 ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche IP: Rattenlebergewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

25347-1-AP bindet in WB, IHC, IP, ELISA FATP5/SLC27A5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FATP5/SLC27A5 fusion protein Ag18446
• Vollständiger Name: solute carrier family 27 (fatty acid transporter), member 5
• Berechnetes Molekulargewicht: 690 aa, 75 kDa
• Beobachtetes Molekulargewicht: 70-75 kDa
• GenBank-Zugangsnummer: BC148807
• Gene symbol: FATP5/SLC27A5
• Gene ID (NCBI): 10998
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SLC27A5, also known as FATP5, ACSVL6, and BAL, is an isozyme of very long-chain acyl-CoA synthetase (VLCS). It is capable of activating very long-chain fatty acids containing 24- and 26-carbons(PMID: 10479480). FATP5 mediates the import of long-chain fatty acids (LCFA) by facilitating their transport across cell membranes (PubMed:20448275). FATP5 is associated with endoplasmic reticulum but not with peroxisomes, and its primary role is in fatty acid elongation or complex lipid synthesis. FATP5 is exclusively expressed by the liver and localized to the basal plasma membrane of hepatocytes (PMID:16618416).

Publikationen

Species	Application	Title
human	WB,IHC	Front Oncol; FATP5 modulates biological activity and lipid metabolism in prostate cancer through the TEAD4-mediated Hippo signaling; Authors - Shenyang Liu