FATP5/SLC27A5 Rekombinanter Antikörper

FATP5/SLC27A5 Rekombinant Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

241884H2

Kat-Nr. : 84557-1-RR

Synonyme

FATP5, SLC27A5, 241884H2, ACSB, ACSVL6

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  WB analysis using 84557-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84557-1-RR (SLC27A5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 84557-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84557-1-RR (FATP5/SLC27A5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 84557-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84557-1-RR (FATP5/SLC27A5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse liver using 84557-1-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using FATP5/SLC27A5 antibody (84557-1-RR, Clone: 241884H2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Mauslebergewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

84557-1-RR bindet in WB, IHC, IF-P, ELISA FATP5/SLC27A5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: FATP5/SLC27A5 fusion protein Ag18276
• Vollständiger Name: solute carrier family 27 (fatty acid transporter), member 5
• Berechnetes Molekulargewicht: 690 aa, 75 kDa
• Beobachtetes Molekulargewicht: 70-75 kDa
• GenBank-Zugangsnummer: BC148807
• Gene symbol: FATP5/SLC27A5
• Gene ID (NCBI): 10998
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SLC27A5, also known as FATP5, ACSVL6, and BAL, is an isozyme of very long-chain acyl-CoA synthetase (VLCS). It is capable of activating very long-chain fatty acids containing 24- and 26-carbons(PMID: 10479480). FATP5 mediates the import of long-chain fatty acids (LCFA) by facilitating their transport across cell membranes (PubMed:20448275). FATP5 is associated with endoplasmic reticulum but not with peroxisomes, and its primary role is in fatty acid elongation or complex lipid synthesis. FATP5 is exclusively expressed by the liver and localized to the basal plasma membrane of hepatocytes (PMID:16618416).

Publikationen

Species	Application	Title
mouse	WB	Nat Commun; Hepatic AKAP1 deficiency exacerbates diet-induced MASLD by enhancing GPAT1-mediated lysophosphatidic acid synthesis; Authors - Linjie He