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FATS Polyklonaler Antikörper

FATS Polyklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30190-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of various lysates using FATS Polyclonal antibody (30190-1-AP)

    WB analysis using 30190-1-AP (same clone as 30190-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30190-1-AP (FATS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30190-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using FATS Polyclonal antibody (30190-1-AP)

    IF Staining of A431 using 30190-1-AP (same clone as 30190-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using FATS antibody (30190-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 30190-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

30190-1-PBS bindet in WB, IF/ICC, Indirect ELISA FATS und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen FATS fusion protein Ag32956
Vollständiger Name chromosome 10 open reading frame 90
Berechnetes Molekulargewicht78 kDa
Beobachtetes Molekulargewicht 67-77 kDa
GenBank-ZugangsnummerNM_001004298.2
Gene symbol FATS/C10orf90
Gene ID (NCBI) 118611
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FATS, also known as centrosomal protein C10orf90, bA422P15.2, belongs to a novel class of enzymes that acts as an E2-E3 hybrid enzyme for assembly of polyubiquitin chains. FATS is an E2-independent ubiquitin ligase that stabilizes p53 and promotes its activation in response to DNA damage (PMID: 24240685). FATS acts as a p53/TP53 activator by inhibiting MDM2 binding to p53/TP53 and stimulating non-proteolytic polyubiquitination of p53/TP53.