FBF1 Polyklonaler Antikörper

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• KD/KO Validated

FBF1 Polyklonal Antikörper für IF, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

22

Kat-Nr. : 11531-1-AP

Synonyme

Alb, albatross, Fas (TNFRSF6) binding factor 1, FBF 1, FBF1, FBF1 Ens, HCG1989313, isoform CRA_b, KIAA1863

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Galerie der Validierungsdaten

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  IF Staining of A549 using 11531-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using FBF1 antibody (11531-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HUVEC using 11531-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using FBF1 antibody (11531-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: A549-Zellen, HUVEC-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 19 publications below
• IP: See 1 publications below

Produktinformation

11531-1-AP bindet in WB, IP, IHC, IF, ELISA FBF1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FBF1 fusion protein Ag2106
• Vollständiger Name: Fas (TNFRSF6) binding factor 1
• Berechnetes Molekulargewicht: 1133 aa, 125 kDa
• Beobachtetes Molekulargewicht: 130 kDa
• GenBank-Zugangsnummer: BC023549
• Gene symbol: FBF1
• Gene ID (NCBI): 85302
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FBF1, also named as ALB and KIAA1863, is keratin-binding protein required for epithelial cell polarization. It is involved in apical junction complex (AJC) assembly via its interaction with PARD3. FBF1 is required for ciliogenesis. FBF1 has six isoforms with MW 125-127 kDa, 99 kDa, 69 kDa and 55 kDa. This antibody is induced by N-terminal (20-347aa) of FBF1. It recognizes all the six isoforms.

Publikationen

Species	Application	Title
human	WB,IF	Nat Commun; A stress-induced cilium-to-PML-NB route drives senescence initiation; Authors - Xiaoyu Ma
human	IF	Sci Adv; Ciliopathy protein HYLS1 coordinates the biogenesis and signaling of primary cilia by activating the ciliary lipid kinase PIPKIγ.; Authors - Chuan Chen
human	IF	Nat Commun; Axoneme polyglutamylation regulated by Joubert syndrome protein ARL13B controls ciliary targeting of signaling molecules.; Authors - Kai He
human	IF	Nat Commun; A distal centriolar protein network controls organelle maturation and asymmetry.; Authors - Lei Wang
human	IF,WB	Nat Commun; TALPID3 and ANKRD26 selectively orchestrate FBF1 localization and cilia gating.; Authors - Hao Yan
human	IF	Nat Commun; Phosphatidylinositol phosphate kinase PIPKIγ and phosphatase INPP5E coordinate initiation of ciliogenesis.; Authors - Qingwen Xu