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FBL Polyklonaler Antikörper
FBL Polyklonal Antikörper für WB, IF/ICC, FC (Intra), IP, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus, Ratte
Anwendung
WB, IF/ICC, FC (Intra), IP, RIP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 16021-1-AP
Synonyme
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | A375-Zellen, Mauslebergewebe |
Erfolgreiche IP | A375-Zellen |
Erfolgreiche Detektion in IF/ICC | HepG2-Zellen |
Erfolgreiche Detektion in FC (Intra) | HepG2-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
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Western Blot (WB) | WB : 1:1000-1:6000 |
Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunfluoreszenz (IF)/ICC | IF/ICC : 1:400-1:1600 |
Durchflusszytometrie (FC) (INTRA) | FC (INTRA) : 0.4 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
KD/KO | See 3 publications below |
WB | See 22 publications below |
IF | See 11 publications below |
IP | See 1 publications below |
RIP | See 4 publications below |
Produktinformation
16021-1-AP bindet in WB, IF/ICC, FC (Intra), IP, RIP, ELISA FBL und zeigt Reaktivität mit human, Maus, Ratten
Getestete Reaktivität | human, Maus, Ratte |
In Publikationen genannte Reaktivität | human, Maus |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Polyklonal |
Typ | Antikörper |
Immunogen | FBL fusion protein Ag8841 |
Vollständiger Name | fibrillarin |
Berechnetes Molekulargewicht | 321 aa, 34 kDa |
Beobachtetes Molekulargewicht | 34 kDa |
GenBank-Zugangsnummer | BC019260 |
Gene symbol | FBL |
Gene ID (NCBI) | 2091 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Antigen-Affinitätsreinigung |
Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Protokolle
PRODUKTSPEZIFISCHE PROTOKOLLE | |
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WB protocol for FBL antibody 16021-1-AP | Protokoll herunterladen |
IHC protocol for FBL antibody 16021-1-AP | Protokoll herunterladenl |
IF protocol for FBL antibody 16021-1-AP | Protokoll herunterladen |
IP protocol for FBL antibody 16021-1-AP | Protokoll herunterladen |
STANDARD-PROTOKOLLE | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Mol Cell lncRNAs maintain the functional phase state of nucleolar prion-like protein to facilitate rRNA processing | ||
Mol Cell LARP7-Mediated U6 snRNA Modification Ensures Splicing Fidelity and Spermatogenesis in Mice.
| ||
Nucleic Acids Res GPATCH4 functions as a regulator of nucleolar R-loops in hepatocellular carcinoma cells | ||
Breast Cancer Res U2AF2-SNORA68 promotes triple-negative breast cancer stemness through the translocation of RPL23 from nucleoplasm to nucleolus and c-Myc expression | ||
Cell Biol Toxicol SNORD17-mediated KAT6B mRNA 2'-O-methylation regulates vasculogenic mimicry in glioblastoma cells |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Tatyana (Verified Customer) (03-11-2023) | Very strong, specific nucleolar signal in ICC. 4% PFA fixation followed by methanol permeabilization; antibody prepared in 5% goat serum in PBST; 2 h RT incubation (no blocking step).
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FH WEI (Verified Customer) (03-12-2020) | Good, strong band for WB and correct staining for cardiomyocytes.
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FH Lauren (Verified Customer) (02-20-2020) | Fixations tested include 2% and 4% formaldehyde for 20 minutes, and -20'C methanol for 10 minutes. Antibody works in all three fixations, but 4% formaldehyde gave the cleanest signal.
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FH Baptiste (Verified Customer) (09-05-2019) | This antibody works well in western blot
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