FBL Monoklonaler Antikörper

FBL Monoklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

CloneNo.

3A9E8

Kat-Nr. : 66985-1-PBS

Synonyme

FLRN, fibrillarin, FIB1, FIB, EC:2.1.1.-

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  WB analysis using 66985-1-Ig (same clone as 66985-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66985-1-Ig (FBL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66985-1-PBS in a different storage buffer formulation.

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  WB analysis using 66985-1-Ig (same clone as 66985-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66985-1-Ig (FBL antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66985-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 66985-1-Ig (same clone as 66985-1-PBS)

  IP result of anti-FBL (IP:66985-1-Ig, 5ug; Detection:66985-1-Ig 1:1000) with HeLa cells lysate 1600 ug. This data was developed using the same antibody clone with 66985-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66985-1-Ig (same clone as 66985-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66985-1-Ig (FBL antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66985-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66985-1-Ig (same clone as 66985-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66985-1-Ig (FBL antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66985-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66985-1-Ig (same clone as 66985-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FBL antibody (66985-1-Ig, Clone: 3A9E8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), Alpha Tubulin antibody (11224-1-AP, red). This data was developed using the same antibody clone with 66985-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66985-1-Ig (same clone as 66985-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FBL antibody (66985-1-Ig, Clone: 3A9E8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), Alpha Tubulin antibody (11224-1-AP, red). This data was developed using the same antibody clone with 66985-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66985-1-Ig (same clone as 66985-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FBL antibody (66985-1-Ig, Clone: 3A9E8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66985-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 66985-1-Ig (same clone as 66985-1-PBS)

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human FBL (66985-1-Ig, Clone:3A9E8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66985-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 66985-1-Ig (same clone as 66985-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug FBL Monoclonal antibody (66985-1-Ig, Clone:3A9E8) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66985-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66985-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), IP, ELISA FBL und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: FBL fusion protein Ag8841
• Vollständiger Name: fibrillarin
• Berechnetes Molekulargewicht: 321 aa, 34 kDa
• Beobachtetes Molekulargewicht: 34 kDa
• GenBank-Zugangsnummer: BC019260
• Gene symbol: FBL
• Gene ID (NCBI): 2091
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fibrillarin (Fbl) is an essential nucleolar protein that plays an essential role in ribosome biogenesis and more particularly in the methylation of ribosomal RNAs and rDNA histones. FBL is highly expressed in various cancers and correlates with poor survival outcomes in cancer patients,such as hepatocellular carcinoma(PMID: 33376525). Knockdown of FBL sensitizes tumor cells and xenografts to DNA crosslinking agents, and leads to homologous recombination-mediated DNA repair defects(PMID: 37489617).