FBLIM1 Polyklonaler Antikörper

FBLIM1 Polyklonal Antikörper für IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 13349-1-AP

Synonyme

CAL, FBLIM1, FBLP 1, FBLP1, filamin binding LIM protein 1, MIG2 interacting protein, Migfilin, RP11 169K16.5

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Galerie der Validierungsdaten

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  WB analysis of human heart using 13349-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 13349-1-AP (FBLIM1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13349-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13349-1-AP (FBLIM1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 13349-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13349-1-AP (FBLIM1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 13349-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13349-1-AP (FBLIM1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 13349-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13349-1-AP (FBLIM1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 13349-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 13349-1-AP (FBLIM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 13349-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 13349-1-AP (FBLIM1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human lung cancer using 13349-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 13349-1-AP (FBLIM1 antibody) at dilution of 1:100 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

13349-1-AP bindet in IHC, IF, ELISA FBLIM1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FBLIM1 fusion protein Ag3907
• Vollständiger Name: filamin binding LIM protein 1
• Berechnetes Molekulargewicht: 373 aa, 41 kDa
• Beobachtetes Molekulargewicht: 45-48 kDa
• GenBank-Zugangsnummer: BC019895
• Gene symbol: FBLIM1
• Gene ID (NCBI): 54751
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FBLIM1, also known as CAL, Mig-2-interacting protein or Migfilin, is a cytoplasmic protein that belongs to the LIM superfamily. FBLIM1 is a protein found in cell-cell and cell-ECM connections where it co-localizes with FLNA/C and FLNB. FBLIM1 was found to bind directly to FLNA/C and to be an important regulator of cell shape and motility. FBLIM1 exerts its influence on cellular functions by interacting with various binding partners; FLN via its N- terminal domain, VASP and Src via its proline-rich region, and kindlin-2 and the cardiac transcription factor, CSX/NKX2-5 via its C-terminal LIM domains. Three isoforms exist for FBLIM1 due to alternative splicing events, namely FBLP-1A, FBLP-1 and FBLP-1B. FBLIM1 serves as an anchoring site for cell-ECM adhesion proteins and filamin-containing actin filaments. It is associated with actin stress fiber at cell-ECM focal adhesion sites. FBLP-1A and FBLP-1B are recruited and localized at actin stress fibers and clustered at cell-EMC adhesion sites through interaction with PLEKHC1. FBLP-1 is localized at actin stress fibers. FBLIM1 is implicated in cell shape modulation (spreading) and motility. FBLIM1 participate in the regulation of filamin-mediated cross-linking and stabilization of actin filaments. It may also regulate the assembly of filamin-containing signaling complexes that control actin assembly. In addition, FBLIM1 is capable of translocating to the nucleus and regulating gene expression. This antibody is a rabbit polyclonal antibody raised against full length human FBLIM1 antigen.

Publikationen

Species	Application	Title
human	IF	J Cell Sci; Functional differences between kindlin-1 and kindlin-2 in keratinocytes.; Authors - Bandyopadhyay Aditi A