Fibrillin 1 Rekombinanter Antikörper

Fibrillin 1 Rekombinant Antikörper für IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

240960E11

Kat-Nr. : 83836-2-PBS

Synonyme

FBN1, 240960E11, Asprosin, FBN, Fibrillin-1

---

• 

  Sandwich ELISA standard curve of MP00807-1

  Sandwich ELISA standard curve of MP00807-1, Human Fibrillin 1/FBN1 Recombinant Matched Antibody Pair - PBS only. 83836-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag30283. 83836-1-PBS was HRP conjugated as the detection antibody. Range: 62.5-4000 pg/mL

• 

  Sample test of MP00807-1

  Serum of sixteen individual healthy human donors was measured. The human Fibrillin 1/FBN1 concentration of detected samples was determined to be 117.73 ng/mL with a range of 43.48 - 246.44 ng/mL.

• 

  IF Staining of HeLa using 83836-2-RR (same clone as 83836-2-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Fibrillin 1 antibody (83836-2-RR, Clone: 240960E11 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83836-2-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP00807-1

  Cytometric bead array standard curve of MP00807-1, Fibrillin 1/FBN1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83836-2-PBS. Detection antibody: 83836-1-PBS. Standard: Ag30283. Range: 0.313-40 ng/mL.

• 

  Affinity and Kinetic Characterization of 83836-2-PBS

  Biolayer interferometry (BLl) kinetic assay of 83836-2-PBS against Human Fibrillin 1 was performed. The affinity constant is 0.848 nM.

• 

  Affinity and Kinetic Characterization of 83836-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83836-2-RR against Human Fibrillin 1 were performed. The affinity constant is 0.103 nM.

---

Geprüfte Anwendungen

Produktinformation

83836-2-PBS bindet in IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Fibrillin 1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Fibrillin 1 fusion protein Ag30283
• Vollständiger Name: fibrillin 1
• GenBank-Zugangsnummer: BC146854
• Gene symbol: Fibrillin 1
• Gene ID (NCBI): 2200
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fibrillin-1 belongs to the fibrillin family. Fibrillins are structural components of 10-12 nm extracellular calcium-binding microfibrils, which occur either in association with elastin or in elastin-free bundles. Fibrillin-1-containing microfibrils provide long-term force bearing structural support. Defects in Fibrillin-1 are a cause of Marfan syndrome (MFS), isolated ectopia lentis (EL), Weill-Marchesani syndrome autosomal dominant (ADWMS), Shprintzen-Goldberg craniosynostosis syndrome (SGS) or MASS syndrome.