FBP1 Polyklonaler Antikörper

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• KD/KO Validated

FBP1 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12842-1-PBS

Synonyme

Liver FBPase, Fructose-1,6-bisphosphatase 1, FBPase 1, FBP, EC:3.1.3.11

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  WB analysis of MCF-7 using 12842-1-AP (same clone as 12842-1-PBS)

  WB result of FBP1 antibody (12842-1-AP; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FBP1 transfected MCF-7 cells. This data was developed using the same antibody clone with 12842-1-PBS in a different storage buffer formulation.

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  WB analysis using 12842-1-AP (same clone as 12842-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12842-1-AP (FBP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12842-1-PBS in a different storage buffer formulation.

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  WB analysis of human liver using 12842-1-AP (same clone as 12842-1-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 12842-1-AP (FBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12842-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 12842-1-AP (same clone as 12842-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12842-1-AP (FBP1 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 12842-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 12842-1-AP (same clone as 12842-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12842-1-AP (FBP1 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 12842-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 12842-1-AP (same clone as 12842-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using FBP1 antibody (12842-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 12842-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

12842-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA FBP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FBP1 fusion protein Ag3837
• Vollständiger Name: fructose-1,6-bisphosphatase 1
• Berechnetes Molekulargewicht: 338 aa, 37 kDa
• Beobachtetes Molekulargewicht: 37-40 kDa
• GenBank-Zugangsnummer: BC012927
• Gene symbol: FBP1
• Gene ID (NCBI): 2203
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FBP1 (Fructose-1,6-bisphosphatase 1) is also named as FBP and belongs to the FBPase class 1 family. It catalyzes the hydrolysis of fructose-1,6 bisphosphate to fructose-6-phosphate and inorganic phosphate. This reaction is an important regulatory site of gluconeogenesis. Defects in FBP1 are the cause of fructose-1,6-bisphosphatase deficiency (FBPD) (PMID:12126934).