FBP1 Monoklonaler Antikörper

FBP1 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2G4E4

Kat-Nr. : 68446-1-PBS

Synonyme

FBP, FBP1, FBPase 1, fructose 1,6 bisphosphatase 1

---

• 

  WB analysis using 68446-1-Ig (same clone as 68446-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68446-1-Ig (FBP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68446-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 68446-1-Ig (same clone as 68446-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 68446-1-Ig (FBP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68446-1-PBS in a different storage buffer formulation.

• 

  WB analysis of T-47D using 68446-1-Ig (same clone as 68446-1-PBS)

  T-47D cells were subjected to SDS PAGE followed by western blot with 68446-1-Ig (FBP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68446-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig liver using 68446-1-Ig (same clone as 68446-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 68446-1-Ig (FBP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68446-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 68446-1-Ig (same clone as 68446-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68446-1-Ig (FBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68446-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 68446-1-Ig (same clone as 68446-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68446-1-Ig (FBP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68446-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 68446-1-Ig (same clone as 68446-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using FBP1 antibody (68446-1-Ig, Clone: 2G4E4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68446-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

68446-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA FBP1 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: FBP1 fusion protein Ag3837
• Vollständiger Name: fructose-1,6-bisphosphatase 1
• Berechnetes Molekulargewicht: 338 aa, 37 kDa
• Beobachtetes Molekulargewicht: 37-40 kDa
• GenBank-Zugangsnummer: BC012927
• Gene symbol: FBP1
• Gene ID (NCBI): 2203
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FBP1 (Fructose-1,6-bisphosphatase 1) is also named as FBP and belongs to the FBPase class 1 family. It catalyzes the hydrolysis of fructose-1,6 bisphosphate to fructose-6-phosphate and inorganic phosphate. This reaction is an important regulatory site of gluconeogenesis. Defects in FBP1 are the cause of fructose-1,6-bisphosphatase deficiency (FBPD) (PMID:12126934).