FBX15 Polyklonaler Antikörper

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FBX15 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13024-1-PBS

Synonyme

FBXO15, F-box only protein 15, F box protein 15, F box only protein 15

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  WB analysis of A2780 using 13024-1-AP (same clone as 13024-1-PBS)

  A2780 cells were subjected to SDS PAGE followed by western blot with 13024-1-AP (FBX15 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13024-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse ovary using 13024-1-AP (same clone as 13024-1-PBS)

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 13024-1-AP (FBX15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13024-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 13024-1-AP (same clone as 13024-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 13024-1-AP (FBX15 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13024-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

13024-1-PBS bindet in WB, IHC, Indirect ELISA FBX15 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FBX15 fusion protein Ag3564
• Vollständiger Name: F-box protein 15
• Berechnetes Molekulargewicht: 434 aa, 49 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC029579
• Gene symbol: FBX15
• Gene ID (NCBI): 201456
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

F-box proteins have been shown to be critical for the ubiquitin-mediated degradation of cellular regulatory proteins, and they are a family of eukaryotic proteins characterized by an approximately 40 amino acid motif. SCF complex, a class of ubiquitin ligases, consists of invariable components, Skp1 and Cullin, and variable components of F-box proteins, which have a primary role in determining substrate specificity. The F-box proteins are divided into 3 classes: FBWs containing WD-40 domains, FBLs containing leucine-rich repeats, and FBXs containing either different protein-protein interaction modules or no recognizable motifs. FBOX15 (or FBX15) belongs to the FBXs class, and 2 isoforms produced by alternative splicing has been described. This antibody is expected detect both isoforms.