FBXO2 Polyklonaler Antikörper

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• KD/KO Validated

FBXO2 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(11)

Kat-Nr. : 14590-1-AP

Synonyme

FBG1, F-box only protein 2, Fbs1, FBX2, NFB42

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  WB analysis using 14590-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14590-1-AP (FBXO2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 14590-1-AP

  SH-SY5Y lysates were subjected to SDS PAGE followed by western blot with 14590-1-AP (FBXO2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 14590-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14590-1-AP (FBXO2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 14590-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14590-1-AP (FBXO2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 14590-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain using 14590-1-AP (FBXO2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of rat brain using 14590-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain using 14590-1-AP (FBXO2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of rat cerebellum using 14590-1-AP

  Immunohistochemical analysis of paraffin-embedded rat cerebellum using 14590-1-AP (FBXO2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of rat cerebellum using 14590-1-AP

  Immunohistochemical analysis of paraffin-embedded rat cerebellum using 14590-1-AP (FBXO2 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SH-SY5Y-Zellen, Caco-2-Zellen, HCT 116-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe, Rattenhirngewebe, Ratten-Cerebellum-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 9 publications below
• IHC: See 6 publications below
• IF: See 3 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below

Produktinformation

14590-1-AP bindet in WB, IHC, IF, IP, CoIP, ELISA FBXO2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FBXO2 fusion protein Ag6122
• Vollständiger Name: F-box protein 2
• Berechnetes Molekulargewicht: 33 kDa
• Beobachtetes Molekulargewicht: 33-40 kDa
• GenBank-Zugangsnummer: BC025233
• Gene symbol: FBXO2
• Gene ID (NCBI): 26232
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

F-box only protein 2 (FBXO2), also known as FBG1 or Fbs1, is a cytoplasmic protein and ubiquitin ligase with high-mannose glycoprotein specificity. FBXO2 plays a significant role in regulating normal and abnormal neuron functions as a ubiquitin-mediated degrader of several neuron glycoproteins. In addition, it has been reported that FBXO2 targets insulin receptors for degradation by ubiquitin, thereby modulating insulin signaling. Recently, emerging evidence demonstrated that FBXO2 was also strongly associated with the occurrence and development of malignant tumors, including gastric cancer and colorectal cancer. The overexpression of FBXO2 significantly promotes PTC cell proliferation (PMID: 39343799). Lactic acid mediates the selective loading of FBXO2 into SEVs through SORBS3/FLOT1, thereby inducing hepatocyte apoptosis, which is an important mechanism of liver fibrosis caused by myogenic SEVs (PMID: 39879982).

Publikationen

Species	Application	Title
human	WB	Sci Rep; Quantitative proteomics by SWATH-MS reveals sophisticated metabolic reprogramming in hepatocellular carcinoma tissues.; Authors - Yanyan Gao
human	WB,IHC	Biochem Biophys Res Commun; FBXO2, a novel marker for metastasis in human gastric cancer.; Authors - Sun Xu
human	WB	Front Med (Lausanne); Follicular dendritic cell differentiation is associated with distinct synovial pathotype signatures in rheumatoid arthritis; Authors - Mohey Eldin M El Shikh ; KD Validated
mouse	WB,IHC,CoIP	Nat Commun; Inhibition of histone methyltransferase Smyd3 rescues NMDAR and cognitive deficits in a tauopathy mouse model; Authors - Jamal B Williams
mouse,human	WB,IP,IHC,IF	World J Gastroenterol; F-box only protein 2 exacerbates non-alcoholic fatty liver disease by targeting the hydroxyl CoA dehydrogenase alpha subunit; Authors - Zhi Liu ; KD Validated
mouse	IF	Stem Cells; Early Wnt Signaling Activation Promotes Inner Ear Differentiation via Cell Caudalization in Mouse Stem Cell-Derived Organoids; Authors - Pei-Ciao Tang