FBXO21 Polyklonaler Antikörper

FBXO21 Polyklonal Antikörper für IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 17648-1-AP

Synonyme

F box only protein 21, F box protein 21, F-box only protein 21, FBX21, FBXO 21

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  IP experiment of rat brain using 17648-1-AP

  IP result of anti-FBXO21 (IP:17648-1-AP, 4ug; Detection:17648-1-AP 1:500) with rat brain tissue lysate 1720 ug.

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  IP experiment of A549 using 17648-1-AP

  IP result of anti-FBXO21 (IP:17648-1-AP, 4ug; Detection:17648-1-AP 1:500) with A549 cells lysate 880 ug.

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  IF Staining of A549 using 17648-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using FBXO21 antibody (17648-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche IP: A549-Zellen, Rattenhirngewebe
• Erfolgreiche Detektion in IF/ICC: A549-Zellen

Empfohlene Verdünnung

• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

17648-1-AP bindet in IF/ICC, IP, ELISA FBXO21 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FBXO21 fusion protein Ag11882
• Vollständiger Name: F-box protein 21
• Berechnetes Molekulargewicht: 537 aa, 63 kDa
• Beobachtetes Molekulargewicht: 75 kDa
• GenBank-Zugangsnummer: BC034045
• Gene symbol: FBXO21
• Gene ID (NCBI): 23014
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FBXO21 (F-box protein 21) is a member of the F-box protein family and serves as the substrate recognition component of the SCF (Skp1-Cullin-F-box) E3 ubiquitin ligase complex. In AML cells, FBXO21 can ubiquitinate p85α, a regulatory subunit of the PI3K pathway, leading to its degradation. This reduces PI3K signaling, promotes p85α dimerization, and activates ERK, thereby influencing cell proliferation and survival. Silencing FBXO21 in AML cells induces differentiation, inhibits tumor progression, and increases sensitivity to chemotherapeutic agents. FBXO21 can regulate immune responses by ubiquitinating and degrading proteins like ASK1 through pro-inflammatory cytokine pathways. It can also ubiquitinate p85α, affecting CXCL10 expression and modulating immune cell infiltration and anti-tumor activity. In renal cell carcinoma, overexpression of FBXO21 significantly suppresses ccRCC cell proliferation and metastasis both in vitro and in vivo. It may be related to the stromal score and the infiltration levels of immune cells associated with prognosis. Additionally, FBXO21 overexpression increases the expression of key molecules in the CREB pathway.