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FBXO38 Rekombinanter Antikörper

FBXO38 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

240513A4

Kat-Nr. : 83509-5-PBS

Synonyme

SP329, MOKA, Fbx38, F box protein 38, F box only protein 38

Filter:
  • Western Blot (WB) analysis of various lysates using FBXO38 Recombinant antibody (83509-5-RR)

    WB analysis using 83509-5-RR (same clone as 83509-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83509-5-RR (FBXO38 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83509-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using FBXO38 Recombinant antibody (83509-5-RR)

    IF Staining of HepG2 using 83509-5-RR (same clone as 83509-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FBXO38 antibody (83509-5-RR, Clone: 240513A4 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83509-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83509-5-RR

    Affinity and Kinetic Characterization of 83509-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83509-5-RR against Human FBXO38 were performed. The affinity constant is 0.233 nM.

Geprüfte Anwendungen

Produktinformation

83509-5-PBS bindet in WB, IF/ICC, ELISA FBXO38 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen FBXO38 fusion protein Ag35444
Vollständiger Name F-box protein 38
Berechnetes Molekulargewicht134kDa，1188aa
Beobachtetes Molekulargewicht125kDa, 140 kDa, 150kDa
GenBank-ZugangsnummerXM_005268513.1
Gene symbol FBXO38
Gene ID (NCBI) 81545
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

F-box-containing protein 38 (FBXO38), a member of the F-box family, was initially discovered as a co-activator of the transcription factor Krüppel-like factor 7 (KLF7) (PMID: 14729953). FBXO38 acts as a substrate-binding receptor for multi-subunit ubiquitin ligase SKP1-CUL1-FBXO38 (PMID: 35769260) . FBXO38 is an E3 ligase of PD-1 that mediates Lys48-linked poly-ubiquitination and subsequent proteasome degradation (PMID: 30487606).