FBXO43 Polyklonaler Antikörper

FBXO43 Polyklonal Antikörper für IF, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 55176-1-AP

Synonyme

EMI2, Endogenous meiotic inhibitor 2, ERP1, F box only protein 43, F box protein 43, Fbp5B, Fbx43, FBXO43

---

Galerie der Validierungsdaten

• 

  WB analysis of HEK-293 using 55176-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 55176-1-AP (FBXO43 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HEK-293 using 55176-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HEK-293 cells using 55176-1-AP (FBXO43 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen
• Erfolgreiche Detektion in IF: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 2 publications below

Produktinformation

55176-1-AP bindet in WB, IHC, IF, ELISA FBXO43 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: F-box protein 43
• Berechnetes Molekulargewicht: 78 kDa
• Beobachtetes Molekulargewicht: ~70 kDa
• GenBank-Zugangsnummer: NM_001029860
• Gene symbol: FBXO43
• Gene ID (NCBI): 286151
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FBXO43, also named as EMI2 and FBP5B, is required to establish and maintain the arrest of oocytes at the second meiotic metaphase until fertilization. It acts by inhibiting the anaphase-promoting complex/cyclosome (APC/C) ubiquitin ligase. FBXO43 recognizes and binds to some phosphorylated proteins and promotes their ubiquitination and degradation. We got ~70 kDa band in our detection.

Publikationen

Species	Application	Title
mouse	WB	Sci Adv; SKP1 drives the prophase I to metaphase I transition during male meiosis.; Authors - Yongjuan Guan
human	WB,IHC	Cancers (Basel); F-Box Protein 43, Stabilized by N6-Methyladenosine Methylation, Enhances Hepatocellular Carcinoma Cell Growth and Invasion via Promoting p53 Degradation in a Ubiquitin Conjugating Enzyme E2 C-Dependent Manner; Authors - Huijun Zhou ; KD Validated
human	WB,IHC	Front Oncol; FBXO43 increases CCND1 stability to promote hepatocellular carcinoma cell proliferation and migration; Authors - Chun-Ming Li