FBXO44 Rekombinanter Antikörper

FBXO44 Rekombinant Antikörper für IF/ICC, FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

IF/ICC, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

230229G7

Kat-Nr. : CL488-82857-2

Synonyme

FBG3, F box/G domain protein 3, F box protein FBX30, F box protein 44, F box only protein 44

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  IF Staining of A431 using CL488-82857-2

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 488 FBXO44 antibody (CL488-82857-2, Clone: 230229G7 ) at dilution of 1:200.

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  FC experiment of A431 using CL488-82857-2

  1x10^6 A431 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Fbxo44 Recombinant Antibody (CL488-82857-2, Clone:230229G7)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: A431-Zellen
• Erfolgreiche Detektion in FC (Intra): A431-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-82857-2 bindet in IF/ICC, FC (Intra) FBXO44 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: FBXO44 fusion protein Ag0977
• Vollständiger Name: F-box protein 44
• Berechnetes Molekulargewicht: 26 kDa
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: BC007832
• Gene symbol: FBXO44
• Gene ID (NCBI): 93611
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FBXO44 is a member of the F-box protein family that shares a function as substrate recognition factors for the SKP1-CUL1-F-box (SCF)-type ubiquitin ligase. Several human FBXs (FBXO2, FBXO6, FBXO17, FBXO27, and FBXO44) share a conserved G domain (also known as FBA domain), which is responsible for recognizing N-glycan moieties by most FBXs in this group except FBXO44. (PMID: 25970626, PMID: 23086937)