FBXO5 Polyklonaler Antikörper

FBXO5 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 10872-1-AP

Synonyme

Early mitotic inhibitor 1, EMI1, F box only protein 5, F box protein 5, FBX5, FBXO5

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 10872-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10872-1-AP (FBXO5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human ovary using 10872-1-AP

  human ovary tissue were subjected to SDS PAGE followed by western blot with 10872-1-AP (FBXO5 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 10872-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10872-1-AP (FBXO5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10872-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10872-1-AP (FBXO5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10872-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10872-1-AP (FBXO5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 10872-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10872-1-AP (FBXO5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 10872-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 10872-1-AP (FBXO5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human placenta using 10872-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10872-1-AP (FBXO5 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human placenta using 10872-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10872-1-AP (FBXO5 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human placenta using 10872-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10872-1-AP (FBXO5 Antibody) at dilution of 1:50.

• 

  IHC staining of human placenta using 10872-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10872-1-AP (FBXO5 Antibody) at dilution of 1:50.

• 

  IHC staining of human tonsillitis using 10872-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10872-1-AP (FBXO5 Antibody) at dilution of 1:50.

• 

  IHC staining of human tonsillitis using 10872-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10872-1-AP (FBXO5 Antibody) at dilution of 1:50.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, humanes Eierstockgewebe, K-562-Zellen, Mausnierengewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe, humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 6 publications below

Produktinformation

10872-1-AP bindet in WB, IHC, ELISA FBXO5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FBXO5 fusion protein Ag1296
• Vollständiger Name: F-box protein 5
• Berechnetes Molekulargewicht: 54 kDa
• Beobachtetes Molekulargewicht: 56 kDa, 45 kDa
• GenBank-Zugangsnummer: BC018905
• Gene symbol: FBXO5
• Gene ID (NCBI): 26271
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FBXO5, also named as EMI1 and FBX5, is a key regulator of the normal cell cycle. It is a a zinc finger protein expressed by a variety of adult tissues and especially in proliferating Ki-67-positive cells. It binds to the APC activators CDC20 and FZR1/CDH1 to prevent APC activation. FBXO5 can also bind directly to the APC to inhibit substrate-binding.

Publikationen

Species	Application	Title
human	WB	Nature; CRL4AMBRA1 is a master regulator of D-type cyclins.; Authors - Daniele Simoneschi
human	WB	Nat Commun; DNA replication initiation factor RECQ4 possesses a role in antagonizing DNA replication initiation; Authors - Xiaohua Xu
human	WB	J Cell Mol Med; An upstream open reading frame regulates vasculogenic mimicry of glioma via ZNRD1-AS1/miR-499a-5p/ELF1/EMI1 pathway.; Authors - Mo Wang
human	WB	J Cancer Res Clin Oncol; Identification of aberrantly expressed F-box proteins in squamous-cell lung carcinoma.; Authors - Kai Wang ; KD Validated
human	WB	Exp Cell Res; The transcript ENST00000444125 of lncRNA LINC01503 promotes cancer stem cell properties of glioblastoma cells via reducing FBXW1 mediated GLI2 degradation.; Authors - Pan Wei
human	WB	Mol Cell Probes; Exploring the role of FBXO5 in gastric cancer; Authors - Junchang Zhang