FBXO5 Polyklonaler Antikörper

FBXO5 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10872-1-PBS

Synonyme

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  WB analysis of HEK-293 using 10872-1-AP (same clone as 10872-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10872-1-AP (FBXO5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10872-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 10872-1-AP (same clone as 10872-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 10872-1-AP (FBXO5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10872-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 10872-1-AP (same clone as 10872-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10872-1-AP (FBXO5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10872-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 10872-1-AP (same clone as 10872-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 10872-1-AP (FBXO5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10872-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human ovary using 10872-1-AP (same clone as 10872-1-PBS)

  human ovary tissue were subjected to SDS PAGE followed by western blot with 10872-1-AP (FBXO5 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10872-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse kidney using 10872-1-AP (same clone as 10872-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10872-1-AP (FBXO5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10872-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat liver using 10872-1-AP (same clone as 10872-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 10872-1-AP (FBXO5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10872-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 10872-1-AP (same clone as 10872-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10872-1-AP (FBXO5 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 10872-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 10872-1-AP (same clone as 10872-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10872-1-AP (FBXO5 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 10872-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 10872-1-AP (same clone as 10872-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10872-1-AP (FBXO5 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 10872-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 10872-1-AP (same clone as 10872-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10872-1-AP (FBXO5 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 10872-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 10872-1-AP (same clone as 10872-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10872-1-AP (FBXO5 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 10872-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 10872-1-AP (same clone as 10872-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10872-1-AP (FBXO5 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 10872-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

10872-1-PBS bindet in WB, IHC, ELISA FBXO5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FBXO5 fusion protein Ag1296
• Vollständiger Name: F-box protein 5
• Berechnetes Molekulargewicht: 54 kDa
• Beobachtetes Molekulargewicht: 56 kDa, 45 kDa
• GenBank-Zugangsnummer: BC018905
• Gene symbol: FBXO5
• Gene ID (NCBI): 26271
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FBXO5, also named as EMI1 and FBX5, is a key regulator of the normal cell cycle. It is a a zinc finger protein expressed by a variety of adult tissues and especially in proliferating Ki-67-positive cells. It binds to the APC activators CDC20 and FZR1/CDH1 to prevent APC activation. FBXO5 can also bind directly to the APC to inhibit substrate-binding.